We narrowed to 11,938 results for: emb
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Plasmid#49766PurposeExpresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces CerevisiaeDepositorAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only
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GPRC5D-DuET
Plasmid#213298PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LD-RA
Plasmid#209863PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-AsCas12f-WT
Plasmid#204635PurposeExpression of wild-type AsCas12f in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAsCas12f
TagsNLSExpressionMammalianPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~P70a-Bottom-T
Plasmid#87314PurposeExpresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.DepositorInsertSplit-Broccoli system
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR
Plasmid#61777PurposeLuciferase reporter containing the 3'UTR of mouse Smad5DepositorInsertmouse Smad5 3'UTR
UseLuciferaseAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR Age HsSAS-6 ST
Plasmid#46382PurposeFor expression of N-terminal fusions.DepositorInsertSAS-6 (SASS6 Human)
UseGateway CloningAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
XP2 Xwnt-3A
Plasmid#16989DepositorPublicationInsertXwnt-3A (wnt3 Frog)
ExpressionBacterialAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
Bin1 delta SH3
Plasmid#168283PurposeExpress SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBin1 (Bin1 Mouse)
TagsEGFPExpressionMammalianMutationaa 1-404Available sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
iRFP670-PIP5K1A
Plasmid#202749Purposeexpresses fluorescent protein-tagged enzymeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiRFP670:PIP5K1A (PIP5K1A Human)
ExpressionMammalianAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_beta2M (MHC Class I light)
Plasmid#187357PurposeBacterial expression and refolding of gp100 pMHCDepositorInsertbeta2M (MHC Class I light)
ExpressionBacterialPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Expression scaffold for dCas9+sgRNA
Plasmid#171799PurposePICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNADepositorInsertPICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
UseCRISPRAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBBi-Pur GFP-TM
Plasmid#139185PurposeSB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.DepositorInsertGFP-TM
UseTransposonExpressionMammalianMutationfused to membrane anchorPromoterEF1αAvailable sinceMarch 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-Gsdmd-NT C122A
Plasmid#133890PurposeExpresses Gsdmd-NT mutant in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGsdmd-NT
ExpressionMammalianAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-Gsdmd-NT C57A
Plasmid#133888PurposeExpresses Gsdmd-NT mutant in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGsdmd-NT
ExpressionMammalianAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME(S273A)-mRuby2-ST
Plasmid#109128PurposeModified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChroME(S273A)-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorPublicationInsertNum1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pRN3P-NLS-EGFP-Btgh-3NLS
Plasmid#194469PurposeEncodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorPublicationInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionBacterialAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-EF1A-LivePAR(Y107A)-Hygro
Plasmid#176073PurposeEGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassetteDepositorInsertLivePAR (Y107A)
UseLentiviralTagsEGFPExpressionMammalianMutationPoint mutation to convert Try107 to AlaPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only