We narrowed to 932 results for: multisite gateway

1. p5E-Crestin-pro (JDW 1320)

   Plasmid

   #229830

   Purpose

   A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Crestin promoter, c-fos minimal promoter, and b-globin intron
   

   Use

   Gateway entry clone

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-EFS (EF1a core) (JDW 1319)

   Plasmid

   #224474

   Purpose

   Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

   Plasmid

   #224505

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexOP-35SCaMV
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

   Plasmid

   #224506

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-oMDC-LexOP-c-Fos
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-p3E-HyPer7

   Plasmid

   #194289

   Purpose

   Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   Multisite gateway entry clone

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-p3E-GLRX-roGFP2

   Plasmid

   #194288

   Purpose

   Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   GLRX-roGFP2
   

   Use

   Multisite gateway entry clone

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

   Plasmid

   #132926

   Purpose

   pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Insert

   Luc2P
   

   Use

   Luciferase and Synthetic Biology; Pmvp gateway en…

   Tags

   PEST destabilization domain

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

   Plasmid

   #229845

   Purpose

   A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lamin nanobody
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

    Plasmid

    #229812

    Purpose

    A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mCherry
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

    Plasmid

    #242587

    Purpose

    A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nlsKateV5, MbBPF-FLAG, FRTNeoFRT
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-EGFP-hs-miR-126 (JDW 1415)

    Plasmid

    #242581

    Purpose

    An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP with an intron containing hs-miR-126 (MIR126 Human)
    

    Expression

    Mammalian

    Promoter

    human EF1a

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

    Plasmid

    #229813

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexAOp-cFos
    

    Use

    Tol2 based expression vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

    Plasmid

    #229817

    Purpose

    A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

    Plasmid

    #229820

    Purpose

    An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid

    #229824

    Purpose

    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

    Plasmid

    #229838

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexAOp
    

    Use

    Tol2 based expression vector

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-H2B

    Plasmid

    #108883

    Purpose

    Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    H2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
    

    Use

    Multisite gateway

    Mutation

    human amino acid sequence but different codon usa…

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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