We narrowed to 649 results for: pBR322

1. pRF001d

   Plasmid

   #156322

   Purpose

   Expresses heterologous rRNA

   Depositor

   Ahmed Badran

   Article

   Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

   Insert

   rRNA operon, orthogonal-antiRBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT antiRBS engineered to orthogonal antiRBS, PS e…

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNK110d

   Plasmid

   #156321

   Purpose

   Expresses heterologous rRNA

   Depositor

   Ahmed Badran

   Article

   Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

   Insert

   rRNA operon, orthogonal-antiRBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT antiRBS engineered to orthogonal antiRBS, PS e…

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PTG

   Plasmid

   #127198

   Purpose

   Single template U6 promoter minimal size plasmid

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Type

   Empty backbone

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. dSp-Rta 100-175

   Plasmid

   #99667

   Purpose

   Expresses dSp Cas9 fused to truncated RTA activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated RTA activation domain (101-175)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. dSp-Rta 50-175

   Plasmid

   #99665

   Purpose

   Expresses dSp Cas9 fused to truncated RTA activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated RTA activation domain (51-175)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDHJS3 BS+

   Plasmid

   #78252

   Purpose

   One of four plasmids needed to create the double Holliday junction substrate (DHJS).

   Depositor

   Tao-shih Hsieh

   Article

   Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

   Inserts

   loxP
   sequence "B"
   loxP2
   

   Use

   Phagemid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVD518

   Plasmid

   #66158

   Purpose

   Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

   Depositor

   James Kaper

   Article

   Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

   Insert

   tdh1
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pU6-tevopreq1-GG-acceptor

   Plasmid

   #174038

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGDP2 MCR-1

   Plasmid

   #118404

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   MCR-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228362

    Purpose

    mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGDP3 arr

    Plasmid

    #112890

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    arr
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGDP1 fosA

    Plasmid

    #112889

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    fosA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGDP1 stat

    Plasmid

    #112886

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    stat
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC-humanADAR1

    Plasmid

    #198235

    Purpose

    Expresses human ADAR1 in yeast

    Depositor

    Brenda Bass

    Article

    Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

    Insert

    human ADAR1 (ADAR Human)
    

    Use

    Tags

    His10_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

    Plasmid

    #228363

    Purpose

    mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. dead Sa Cas9

    Plasmid

    #138162

    Purpose

    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    dSaCas9
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D10A + N580A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

    Plasmid

    #228364

    Purpose

    mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Enascas12a nuclease and en…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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