We narrowed to 7,394 results for: 11

1. pEasyG3_mic

   Plasmid

   #184920

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PDF1::mCitrine-MBD

   Plasmid

   #115222

   Purpose

   Microtubule visualization

   Depositor

   Jan Traas

   Article

   Armezzani et al Development. 2018 Jun 4;145(11). pii: de

   Insert

   Microtubule binding domain
   

   Tags

   mCitrine

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-REDD1 77-232 (#426)

   Plasmid

   #99921

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 77-232

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TALEN-CCR5R18-CtermQ7

   Plasmid

   #51443

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Q7 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK4a_020

   Plasmid

   #123833

   Purpose

   Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   d4PEST tag
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 Flag-Smad1 (4SP/AP)

   Plasmid

   #14043

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

   Insert

   Smad1 (4SP/AP) (SMAD1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Four Serine to Alanine mutations in the linker re…

   Available Since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

7. MTK5_012

   Plasmid

   #123856

   Purpose

   Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector RE'-A1 Insulator
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATF4 10: 5’ATF4.uORF1.GFP

   Plasmid

   #21857

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   ATF4 (Atf4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   upstream ORF1 (uORF) of ATF4 fused to EGFP

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cx43-sfGFP

    Plasmid

    #70053

    Purpose

    Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    non-monomerized superfolder GFP (V207)

    Mutation

    fused in frame to non-monomerized superfolder GFP

    Promoter

    CMV

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK0_030

    Plasmid

    #123969

    Purpose

    Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    phiC31-hygro-mRuby2PEST landing
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CDK4mut(K35M)-3XFlag-pBabe

    Plasmid

    #73559

    Purpose

    vector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK4mut(K35M)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK3_008

    Plasmid

    #123723

    Purpose

    Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NLS-VP16-EL222
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK7_013

    Plasmid

    #123880

    Purpose

    Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    RA_hAAVS1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EBFP2-Cx43

    Plasmid

    #69020

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    EBFP2

    Promoter

    CMV

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNEXdelta-EGFP-ApAF

    Plasmid

    #13335

    Depositor

    Bong-Kiun Kaang

    Article

    Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

    Insert

    Aplysia activating factor
    

    Tags

    EGFP

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

19. MTK2_004

    Plasmid

    #123699

    Purpose

    Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pPGK1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEasyG3_hph

    Plasmid

    #184919

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge