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pCDNA5_miniTurbo-C12orf49

Plasmid

#155111

Purpose

transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKT0233

Plasmid

#8737

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Tags

13x Myc and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0240

Plasmid

#8739

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Tags

13x Myc and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaq

Plasmid

#246001

Purpose

Express HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnaq (Gnaq Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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plexm-mouse LRRC33

Plasmid

#111601

Purpose

Express mouse LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

mouse LRRC33 (Nrros Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Starts at aa 18

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EAAARx8-CaaX

Plasmid

#155224

Purpose

MPAct with increased search radius

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+Lifeact-eGFP

Plasmid

#128428

Purpose

Contains insert allowing in-vivo visualization of F-actin

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

Lifeact-eGFP

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pOSV810

Plasmid

#126603

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRL1063a

Plasmid

#70687

Purpose

pRL1058 with Vibrio fischeri luxAB for sensing

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1063

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#72867

Purpose

template for N-terminal 3x FLAG tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRB36

Plasmid

#72861

Purpose

template base vector for N-terminal tagging, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for introduction of tags

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSK25

Plasmid

#72853

Purpose

template for C-terminal double-FLAG tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

double Flag

Expression

Insect

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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shha-pCRII

Plasmid

#68962

Purpose

for antisense probe, NotI w/ Sp6

Depositor

David Traver

Article

Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

Insert

shha (shha Zebrafish)

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab4 WT

Plasmid

#37690

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab7 CA

Plasmid

#44825

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Q67L (constitutive active)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
28 pCS plcg 1

Plasmid

#13059

Depositor

Nathan Lawson

Article

Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

Insert

plcg1 (plcg1 Zebrafish)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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p142

Plasmid

#8385

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

LOX I/LOX II

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
p414GPD_Hsp82_CycTER

Plasmid

#61710

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

GPD

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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