We narrowed to 5,023 results for: golden gate

Showing: 3441 - 3460 of 5023 results

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNP2322 [Cloning backbone, mCherry Inversion Activity Reporter]

Plasmid

#247250

Purpose

Cloning backbone for measuring bridge recombinase mediated inversion

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGY037 BSMV-gamma-AmCyan

Plasmid

#234818

Purpose

To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTU2S-a (p15A origin)

Plasmid

#74088

Purpose

Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRDB_270

Plasmid

#231111

Purpose

C>T base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

TadA-CDd-nCas9-SpG-UGI-HA

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-T3G-ERP2

Plasmid

#175188

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTipi2.2b

Plasmid

#252900

Purpose

Golden gate destination vector, allows for expression of recombinant proteins, no N-term tag

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ131A2.0

Plasmid

#99884

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-Dest-BsaI

Plasmid

#200550

Purpose

Mini circle destination vector with BsaI sites for Golden Gate Assembly

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2916 [Cloning backbone, bRNA Landing Pad]

Plasmid

#247254

Purpose

Cloning backbone for cloning bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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TET-ZF37-mCherry-BGH_pKG01502

Plasmid

#246377

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TET-ZF43-TagBFP-BGH_pKG01531

Plasmid

#246378

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK036 [Con-Pro_rpsM-Xantho_PartType5]

Plasmid

#229273

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_rpsM-Xantho_PartType5

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

rpsM Promoter (Xanthobacter flavus GJ10)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCEC-red

Plasmid

#196040

Purpose

Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

Depositor

Paola Branduardi

Article

Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Grna cloning

Expression

Yeast

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-SunTag(10X)-2A-BFP

Plasmid

#227389

Purpose

Adhesion module, dCas9-SunTag

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

dCas9-SunTag

Use

Lentiviral

Mutation

Enzymatically dead Cas9

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNP3614 [pNP3613 - ISCro4 Recombinase (WT)]

Plasmid

#247265

Purpose

Express ISCro4 recombinase

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP3613 - ISCro4 Recombinase (WT)

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2321 (Cloning backbone, bRNA + recombinase]

Plasmid

#247249

Purpose

Cloning backbone for cloning bridge recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5

Plasmid

#233460

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKG1988_pPV1-UbC

Plasmid

#239689

Purpose

BsaI Golden Gate assemby plasmid encoding the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

UbC promoter

Use

Synthetic Biology

Mutation

Removal of BsaI cut sites

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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