We narrowed to 3,637 results for: golden gate

1. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

   Plasmid

   #235305

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   FXN-P2A-mRuby2 (FXN Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

   Plasmid

   #235306

   Purpose

   Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   EGFP-Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

   Plasmid

   #229544

   Purpose

   Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   Oct4(co)-mNeonGreen (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Oct4-mNeonGreen

   Expression

   Mammalian

   Mutation

   codon-optimized OCT4

   Promoter

   TRE12

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

   Plasmid

   #229545

   Purpose

   Lentiviral construct to express rtTA and SKM proteins

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PDG458 eSp(1.1) V3

   Plasmid

   #226964

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HNRNPA1-miRFP670-TRF1

   Plasmid

   #227384

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28:LanIV-Chondromyces

   Plasmid

   #225078

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIV-Chondromyces)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28:LanII-56

   Plasmid

   #225073

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-56)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28:LanI-101

   Plasmid

   #225065

   Purpose

   Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanI-101)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28:LanII-2E

    Plasmid

    #225072

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2E)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28:LanII-2D

    Plasmid

    #225071

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2D)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBS-859

    Plasmid

    #218009

    Purpose

    mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Factor Xa protease cleavage site and Strep-tag II

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMBS-852

    Plasmid

    #218010

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMBS-864

    Plasmid

    #218018

    Purpose

    GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    GST-tag
    

    Use

    Synthetic Biology

    Tags

    TEV protease cleavage site

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMBS-839

    Plasmid

    #217995

    Purpose

    sAP1 protein secretion signal

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Secretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMBS-845

    Plasmid

    #218000

    Purpose

    Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Strep-tag II
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBS-848

    Plasmid

    #218003

    Purpose

    GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    GST-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMBS-849

    Plasmid

    #218004

    Purpose

    8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    8xHis-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBS-850

    Plasmid

    #218005

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBS-857

    Plasmid

    #218007

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge