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pPHAGE_DLD-H450A-C-TAP

Plasmid

#83478

Purpose

Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

H450A

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7WT-VA

Plasmid

#115182

Purpose

Lentiviral transduction and expression of PARK7WT into any mammalian cell

Depositor

Mohan Babu

Publication

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7WT (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Promoter

CMV

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPHAGE-CR-NENF-C-TAP

Plasmid

#128511

Purpose

Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

Depositor

Mohan Babu

Publication

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

NENF (NENF Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Synonymous silent mutations to F81, Y82, G83 and …

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

Plasmid

#128551

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Publication

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TARDBP)

Plasmid

#109323

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

Depositor

Feng Zhang

Publication

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TARDBP Human)

Use

AAV

Promoter

hSyn1

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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UbC-Halo-SFPQY527A

Plasmid

#166947

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

UbC

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Myc-KLC1 delta Tail

Plasmid

#166965

Purpose

Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

KLC1 (1-495aa)

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5AR280H

Plasmid

#166957

Purpose

Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A-R280H (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A-R280H

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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KLC1N343S-Myc

Plasmid

#166963

Purpose

Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1-N343S (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

KLC1-N343S

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

Plasmid

#139096

Purpose

Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Publication

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dxCas9 3.7

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Lentivirus ABE8e-V106W with non-targeting sgRNA

Plasmid

#247668

Purpose

Lentivirus genome encoding ABE8eV106W editor and Sp sgRNA cassette non-targeting control

Depositor

David Liu

Publication

Gao et al Nat Biomed Eng. 2026 Apr 14. doi: 10.103

Insert

ABE8e-V106W and non-targeting sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-tTA

Plasmid

#178516

Purpose

AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

tTA

Use

AAV

Expression

Mammalian

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPHAGE-C20orf24- C20orf24-3’UTR-WT

Plasmid

#128508

Purpose

Lentiviral constitutive expression of C20orf24 under control of its native WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Publication

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

RAB5IF (RAB5IF Human)

Use

Lentiviral

Tags

FLAG and HA tags

Expression

Mammalian

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Publication

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Promoter

hSyn

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Publication

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Promoter

hSyn1

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAV C-terminus ABE8e-V106W targeting Pex1-G844D

Plasmid

#247655

Purpose

AAV genome encoding the C-terminus of ABE8e-V106W editor and Sp sgRNA cassette

Depositor

David Liu

Publication

Gao et al Nat Biomed Eng. 2026 Apr 14. doi: 10.103

Insert

C-terminus ABE8e-V106W editor and Pex1-G844D-targeting sgRNA

Use

AAV and CRISPR

Promoter

CBh

Available since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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AAV N-terminus ABE8e-V106W targeting Pex1-G844D

Plasmid

#247654

Purpose

AAV genome encoding the N-terminus of ABE8e-V106W editor and Sp sgRNA cassette

Depositor

David Liu

Publication

Gao et al Nat Biomed Eng. 2026 Apr 14. doi: 10.103

Insert

N-terminus ABE8e-V106W editor and Pex1-G844D-targeting sgRNA

Use

AAV and CRISPR

Promoter

CBh

Available since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Publication

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-mCre

Plasmid

#178515

Purpose

AAV vector for mCre recombinase

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCre

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-TTBG

Plasmid

#115232

Purpose

Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)

Expression

Mammalian

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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