We narrowed to 3,637 results for: golden gate

1. pYPQ133-STU-Lb

   Plasmid

   #138102

   Purpose

   Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ132-STU-Lb

   Plasmid

   #138099

   Purpose

   Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ144-ZmUbi-pT

   Plasmid

   #138108

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNK5712

   Plasmid

   #219752

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnH3H_v2 - tAOX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ142-ZmUbi

   Plasmid

   #138106

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNK5867

   Plasmid

   #219751

   Purpose

   pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - mcitHispS - tAOX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNK1508

   Plasmid

   #219750

   Purpose

   pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - npgA - tAOX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNK2657

   Plasmid

   #219741

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   hispidin- synthase from Mycena citricolor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218914

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNK3203

    Plasmid

    #219749

    Purpose

    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - mcitHispS - tSV40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218910

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ143-ZmUbi

    Plasmid

    #138107

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ131-STU-As

    Plasmid

    #138094

    Purpose

    Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ134-STU-Fn

    Plasmid

    #138104

    Purpose

    Golden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ131-STU-Fn

    Plasmid

    #138095

    Purpose

    Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ132-STU-Fn

    Plasmid

    #138098

    Purpose

    Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ133-STU-Fn

    Plasmid

    #138101

    Purpose

    Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-U6-sgNT-2-EF1a-LibVec

    Plasmid

    #239593

    Purpose

    expresses non-targeting (NT) guide#2

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    NT (non-targeting guide)
    

    Use

    AAV; To deliver a non-targeting guide (serving as…

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-U6-sgNT-1-EF1a-LibVec

    Plasmid

    #239592

    Purpose

    expresses non-targeting (NT) guide#1

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    NT (non-targeting guide)
    

    Use

    AAV; To deliver a non-targeting guide (serving as…

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge