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pGEX-KG-GST-YAP-S127A

Plasmid

#33053

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

GST

Expression

Mammalian

Mutation

S127A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GdT

Plasmid

#87361

Purpose

Expresses GdT, a dimeric green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GdT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-Tubulin-C-18

Plasmid

#57432

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Tubulin (TUBA1B Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pC-PTP-NEO

Plasmid

#51710

Purpose

contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available Since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_L706-antiSH2-OptoMB-irFP

Plasmid

#159938

Purpose

Opto-Monobody mammalian expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Lentiviral

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα-T391A

Plasmid

#119920

Purpose

Mammalian expression of the tAKARα sensor with T391A mutation

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα-T391A

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.718

Plasmid

#108519

Purpose

PBetI-YFP Reporter. Cho sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBetI-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.1459

Plasmid

#108521

Purpose

P3B5C-YFP Reporter. DHBA sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

P3B5C-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS3

Plasmid

#109287

Purpose

Expresses proteins in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Type

Empty backbone

Expression

Bacterial

Mutation

New promoter/cloning site

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-Cer.zf1

Plasmid

#61389

Purpose

mRNA synthesis of cerulean codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cer.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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258/-2.5CAT

Plasmid

#25431

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter/Enhancer (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
FRP800_insul-(lexA-box)3-PminCYC1-Citrine-TCYC1

Plasmid

#58436

Purpose

Induces Citrine expression from a promoter containing 3 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PPP1CA_Metallophos

Plasmid

#109934

Purpose

Protein expression and purification of PPP1CA_Metallophos

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPP1CA_Metallophos

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM24951

Plasmid

#169088

Purpose

MoClo level 0 Tomato E8 promoter module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Tomato E8 promoter

Use

Synthetic Biology; Moclo cloning vector

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Clover-Lifeact-7

Plasmid

#54551

Purpose

Localization: Actin, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Clover-Lifeact

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAJM.719

Plasmid

#108520

Purpose

PTtg-YFP Reporter. Nar sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTtg-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.711

Plasmid

#108512

Purpose

PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PPhlF-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15

Plasmid

#159593

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT14.Y

Plasmid

#201009

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sYFP2, SpR,StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Expression

Bacterial

Promoter

J23100

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Tags

FRB (T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pXcX-3.6TPH-GAL4p65

Plasmid

#32573

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

Gal4p65

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-CaARG4

Plasmid

#162463

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST1K18ms

Plasmid

#72646

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Article

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available Since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_iRFP670

Plasmid

#62411

Purpose

MXS_chaining vector with iRFP670

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

iRFP670

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_mAzamiGreen

Plasmid

#62404

Purpose

MXS_chaining vector with mAzamiGreen

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mAzamiGreen

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HisB-GGvT

Plasmid

#87362

Purpose

Expresses GGvT, a tandem homodimer green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GGvT

Tags

His-Tag

Expression

Bacterial

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Aac.4

Plasmid

#158406

Purpose

Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT14.G

Plasmid

#201002

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sfTq2-C1

Plasmid

#117932

Purpose

Superfolder mTurquoise2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV-pOTC-3H-GFP

Plasmid

#205725

Purpose

Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-3H-GFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY 3010-02

Plasmid

#36210

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yeast-enhanced (yE) polylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-hphMX4

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pF hPGK mc2 IRES neo

Plasmid

#206236

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_mCherry

Plasmid

#62408

Purpose

MXS_chaining vector with mCherry

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mCherry

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_mKO2

Plasmid

#62406

Purpose

MXS_chaining vector with mKO2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mKO2

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBORF-DBD

Plasmid

#127463

Purpose

ORF filtering plasmid (lambda cI associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK409 (mAID-Nluc BSD)

Plasmid

#121199

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc BSD

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
MXS_rtTA3

Plasmid

#62428

Purpose

MXS_chaining vector with rtTA3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Reverse tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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