Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,092 results for: codon optimized

Showing: 3521 - 3540 of 5092 results

  6. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

    Enlarge

  9. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

    Enlarge

  10. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

    Enlarge

  14. CoV2-N-WT-Hu1

    Plasmid
    #177937
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177937-plasmid-map-sequence-id-348099

    Enlarge

  15. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Pantelis Tsoulfas, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

    Enlarge

  16. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Expression
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

    Enlarge

Showing: 3521 - 3540 of 5092 results