We narrowed to 5,308 results for: pcDNA3.1+

1. mc2 neo

   Plasmid

   #206214

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CENP-B mCherry PP1 gamma

   Plasmid

   #45221

   Depositor

   Michael Lampson, Iain Cheeseman

   Article

   Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

   Insert

   CENP-B mCherry PP1 gamma
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVH72-Tier1-PhCMV-SEAP

   Plasmid

   #169560

   Purpose

   Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven human secreted placental alkaline phosphatase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cenp-B DBD INCENP mCherry

   Plasmid

   #45233

   Purpose

   Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B-DBD INCENP-Δcen mCherry
   

   Use

   Tags

   DNA binding domain of CENP-B and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. aq-CFP

   Plasmid

   #36073

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   G protein subunit alpha q (Gnaq Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted between amino acid 124 and 125

   Promoter

   CMV

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. GFP2-NbV5

   Plasmid

   #201478

   Purpose

   BRET2 detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   GFP2-NbV5
   

   Use

   Tags

   GFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cav1-mRed

   Plasmid

   #12681

   Depositor

   Richard Pagano

   Article

   Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

   Insert

   canine caveolin-1 (CAV1 Canis familiaris)
   

   Use

   Tags

   mRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pGas-miRFP670nano3

   Plasmid

   #184672

   Purpose

   G protein alpha-subunit internally tagged with miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   G protein alpha-s-miRFP670nano3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FLAG-Fos AA

   Plasmid

   #8967

   Depositor

   John Blenis

   Article

   Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

   Insert

   c-Fos (Fos Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S362A, S374A

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

10. C-term Hec1-targeted Aurora B FRET sensor

    Plasmid

    #45222

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    C-term Hec1-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-3xflagGREB1b

    Plasmid

    #112222

    Purpose

    mammalian expression of flag tagged GREB1b

    Depositor

    Craig Burd

    Article

    Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

    Insert

    GREB1b (GREB1 Human)
    

    Use

    Tags

    3x flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA PRAS40 S183A

    Plasmid

    #15674

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 S183A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S183A

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

13. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

14. Septin 11 isoform IV

    Plasmid

    #120320

    Purpose

    Septin 11 isoform IV mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Septin 11 isoform I

    Plasmid

    #120314

    Purpose

    Septin 11 isoform I mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. h-alpha-ENaC-2

    Plasmid

    #83431

    Purpose

    full length human alpha ENaC-2 with flag tag

    Depositor

    Christie Thomas

    Article

    Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

    Insert

    human alpha ENaC isoform 2 (SCNN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDWUER

    Plasmid

    #37094

    Depositor

    David Wang

    Article

    Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

    Insert

    WU polyomavirus early region (WUPyV_gp4, WUPyV_gp5 WU polyomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YFP Gamma 1

    Plasmid

    #36101

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 1 (GNGT1 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-IP3KC

    Plasmid

    #32527

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

    Insert

    IP3KC (ITPKC Human)
    

    Use

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only