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pISH-Neurod1

Plasmid

#74396

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Publication

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Tags

EGFP

Expression

Worm

Promoter

dpy-7

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBS xChordin

Plasmid

#24975

Depositor

Edward De Robertis

Publication

Sasai et al Cell. 1994 Dec 2. 79(5):779-90.

Insert

Chordin (chrd.1.S Frog)

Expression

Bacterial

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pPD96_04

Plasmid

#1502

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, g…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AiP2135 - pAAV-AiE2326m-minBG-iCre(R297T)-BGHpA

Plasmid

#243044

Purpose

AiE2326m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Insert

iCre(R297T)

Use

AAV

Mutation

Mutation in 297th amino acid

Available since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW COX8ASig-Lemon

Plasmid

#239560

Purpose

Expression and mitochondrial localization of Lemon, a pH biosensor

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

Lemon

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry-P2A-PuroR

Plasmid

#239567

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#233538

Purpose

A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Tags

V5

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-ER

Plasmid

#214413

Purpose

ER localization of CFAST fused to Halotag-ER

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Tags

CFAST-Halo

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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APEX2-6xHp

Plasmid

#214411

Purpose

Lipid droplet localization of APEX2-6xHp for electron microscopy

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (APEX2 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-6xHp

Plasmid

#214416

Purpose

Lipid droplet localization of CFAST fused to Halotag-6xHp

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

deletion

Tags

CFAST-Halo

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCL2L1_BH4+Bcl-2

Plasmid

#109993

Purpose

Protein expression and purification of BCL2L1_BH4+Bcl-2

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

BCL2L1_BH4+Bcl-2 (BCL2L1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR1_Hormone-recep

Plasmid

#109906

Purpose

Protein expression and purification of ESR1_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR1_Hormone-recep (ESR1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-OCAM

Plasmid

#136767

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Ncam2

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Ncam2 (Ncam2 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS1964

Plasmid

#82574

Purpose

High copy number plasmid containing Ple305 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple305

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 23, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB102-Citrine-PTS1

Plasmid

#44592

Purpose

Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants

Depositor

Yutaka Kodama

Publication

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Expression

Plant

Promoter

CaMV 35S promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS254

Plasmid

#73721

Purpose

SapTrap Halotag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Halotag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrambled-GFP

Plasmid

#228736

Purpose

GFP expressing scramble of shRNA targeting Miro1

Depositor

Cagla Eroglu

Publication

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Mouse, Rat)

Expression

Mammalian

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Publication

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLPS1-iLID::EGFP::FTH1 (pBS1042)

Plasmid

#185289

Purpose

For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

iLID::eGFP::FTH1

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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