We narrowed to 1,432 results for: golden gate assembly

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GGE255

Plasmid

#120788

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsUp_Lip-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

Plasmid

#170767

Purpose

Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XbaI
M.EcoRI
M.SalI

Expression

Bacterial

Promoter

rhamnose

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

Plasmid

#170768

Purpose

Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XhoI
M.MunI
M.Ocy1ORF8430P

Expression

Bacterial

Promoter

rhamnose

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GGE207

Plasmid

#120792

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown-Mfe

Use

Cloning/assembly plasmid

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA13

Plasmid

#158491

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA7

Plasmid

#158485

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA15

Plasmid

#158493

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA6

Plasmid

#158484

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

D481V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA5

Plasmid

#158483

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA14

Plasmid

#158492

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

D481V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pXZ13lacZalpha

Plasmid

#160229

Purpose

places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

Depositor

Ian Duncan

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGGDestSC+ATG

Plasmid

#49323

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest+

Plasmid

#49319

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p3E_GGWDest-

Plasmid

#49316

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest-

Plasmid

#49318

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pME_GGWDest+

Plasmid

#49321

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGDestSC-ATG

Plasmid

#49322

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p3E_GGWDest+

Plasmid

#49317

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pME_GGWDest-

Plasmid

#49320

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge