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pMM2_11

Plasmid

#183060

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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LD221

Plasmid

#69189

Purpose

Integration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg21-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMM0623

Plasmid

#128998

Purpose

pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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LD214

Plasmid

#69185

Purpose

Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg14-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD205

Plasmid

#69180

Purpose

Integration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg5-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD-G418R (01-21)

Plasmid

#101703

Purpose

G418R in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[G418R (loxP-PAgTEF-kanMX-TAgTEF-loxP)]-CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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LD213

Plasmid

#69184

Purpose

Integration of 2GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg13-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pK3FS-PYK1

Plasmid

#85777

Purpose

N-ICE plasmid pK3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJAID5VY

Plasmid

#165048

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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416-Met25 Kar2 printor HDEL

Plasmid

#32129

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

Printor (KLHL14 Human)

Tags

HDEL and Kar2

Expression

Yeast

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pUPD-Su9MTS (12)

Plasmid

#101691

Purpose

Su9MTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from Neurospora crassa ATP synthase subunit 9 (OR74A)]-AATG

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAV0835

Plasmid

#133512

Purpose

Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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LD224

Plasmid

#69191

Purpose

Integration of 2GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg24-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD218

Plasmid

#69187

Purpose

Integration of 2GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg18-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJAID8Z104

Plasmid

#165056

Purpose

Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPS832

Plasmid

#1357

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pPS833

Plasmid

#1358

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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