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pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

Plasmid

#197961

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-vCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

Plasmid

#197962

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-nCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Lsg-LMNA-1 + 2

Plasmid

#199278

Purpose

nicking sgRNA to induce LMNA (c.1824C > T) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA nick

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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human b1 ecto-pIRES

Plasmid

#113387

Purpose

Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human beta 1 ectodomain

Tags

His tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA6-LRRC38-V5-His

Plasmid

#127820

Purpose

Expression of human LRRC38 in mammalian cells

Depositor

Jiusheng Yan

Article

Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

Insert

LRRC38 (LRRC38 Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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OA-1055J

Plasmid

#200251

Purpose

pBac-U6-gRNA(Intersex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

2 gRNAs targeting Intersex gene

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R

Plasmid

#79063

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5-21R

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version2

Plasmid

#197950

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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a5-ecto-pcDNA31hygro

Plasmid

#113386

Purpose

Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human alpha 5 ectodomain

Tags

Strep II tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pST1374-Lb-Cpf1-NLS

Plasmid

#107680

Purpose

Expresses LbCpf1 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

LbCpf1

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTL-Bag2T-gRNA2

Plasmid

#196253

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 shRNA

Plasmid

#122020

Purpose

Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5-mCherry

Plasmid

#119934

Purpose

Protocadherin gamma C5 expression (mCherry-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-del 50-59

Plasmid

#33065

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 50-59

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSLBPres

Plasmid

#82454

Purpose

Encodes synthesized SLBP that is resistant to SMARTpool siRNA

Depositor

Bharat Ramratnam

Article

Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

Insert

SLBP (SLBP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
Ad-SNRK

Plasmid

#131539

Purpose

Expresses SNRK in mammalian cells

Depositor

Haiyan Xu

Article

Li et al Diabetes. 2013 Jul;62(7):2396-409. doi:

Insert

SNF related kinase (Snrk Mouse)

Use

Adenoviral

Tags

No

Mutation

No

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 shRNA

Plasmid

#122023

Purpose

Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC53

Plasmid

#209962

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

5' Connector (5C1CSN_Tcat+sai)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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XPO6-2Strep

Plasmid

#210356

Purpose

Expresses XPO6 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

XPO6 (XPO6 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TRAM2-2Strep

Plasmid

#210374

Purpose

Expresses TRAM2 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM2 (TRAM2 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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