We narrowed to 8,729 results for: Set

1. pCAG-Beatrix

   Plasmid

   #167528

   Purpose

   Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

   Depositor

   Gian Michele Ratto

   Article

   Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

   Insert

   Beatrix
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TrpC5 scFv [N67/15]

   Plasmid

   #190564

   Purpose

   Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag YFP hNKCC1 (NT17)

   Plasmid

   #49085

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available Since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. peSCBE3-NG

   Plasmid

   #218163

   Purpose

   This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCBE3-NG

   Plasmid

   #218159

   Purpose

   This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / L1111…

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Neuroligin-2 scFv [L107/95]

   Plasmid

   #206764

   Purpose

   Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/95 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

   Plasmid

   #218344

   Purpose

   Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. VGlut2 scFv [N29/29]

   Plasmid

   #182085

   Purpose

   Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Parvalbumin scFv [L114/3]

   Plasmid

   #190504

   Purpose

   Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
   

   Tags

   HA, 6xHis, Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPHKAP scFv [L131/17]

    Plasmid

    #190511

    Purpose

    Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pan-Synapsin scFv [L125/129]

    Plasmid

    #182079

    Purpose

    Mammalian Expression of pan-Synapsin scFv. Derived from hybridoma L125/129.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting pan-Synapsin
    

    Tags

    Sortase, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Parvalbumin scFv [L114/38]

    Plasmid

    #206777

    Purpose

    Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/38 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

    Plasmid

    #113694

    Purpose

    U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HNF4A_P2-2200

    Plasmid

    #31062

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -2200 to -1 of P2 HNF4A promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. HNF4A_P2-285

    Plasmid

    #31060

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid

    #187459

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid

    #187456

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PLL5.0-Anillin ShRNA-Cherry-AnillinFL

    Plasmid

    #187271

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICOZ-SB100X[EF1α-CD19 4-1BB-eGFP]

    Plasmid

    #218343

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLP-PIGA2 (pLP300)

    Plasmid

    #168462

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

    Depositor

    Matthew Maurano

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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