We narrowed to 14,937 results for: pET

1. human EB1 in PET28a for protein expression

   Plasmid

   #39297

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Article

   Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-His tagged nuclear Pif1

   Plasmid

   #65047

   Purpose

   expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

   Depositor

   Jean-Baptiste Boulé, Virgina Zakian

   Article

   Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

   Insert

   Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
   

   Use

   Tags

   histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

   Plasmid

   #136684

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Use

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mTagBFP2-TEV-Strep-His

   Plasmid

   #210824

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mTagBFP2
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET empty polycistronic destination vector (2D)

   Plasmid

   #29774

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 E2 LBD-Tev-E1a-His

   Plasmid

   #232117

   Purpose

   For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   DBT (DBT Human)
   

   Use

   Tags

   6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-(-30)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62372

   Purpose

   Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP-9xGGS-Cre-6xHis
   

   Use

   Tags

   (-30)GFP and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-His6-Sumo-CfaC-MBP

    Plasmid

    #126018

    Purpose

    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-CfaC-MBP
    

    Use

    Tags

    6xHis-SUMO and HA-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AcpP pet29a C-terminal His Tag

    Plasmid

    #75016

    Purpose

    Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    AcpP (acpP E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET28a(+) NRP1 b1b2 (273-586)

    Plasmid

    #162733

    Purpose

    Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1b2 domains (residues 273-586)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Codon optimised

    Promoter

    Available since

    March 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET-15b-hAR-663-919

    Plasmid

    #89083

    Purpose

    bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

    Insert

    hAR ligand binding domain (AR Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 663-919

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28a-His6-Halo-Tev-Keap1

    Plasmid

    #62456

    Purpose

    His6-halo tagged human Keap1 protein for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a His tag-FRB-Venus C

    Plasmid

    #100980

    Purpose

    Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    His-FRB-Venus C
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28C-mCherry-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204403

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge