We narrowed to 11,276 results for: Vars

1. pCMV6-LacZ-BbsI

   Plasmid

   #114671

   Purpose

   For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression

   Depositor

   Lynne Postovit

   Insert

   LacZ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Lac promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB602

   Plasmid

   #22071

   Depositor

   Emmanuelle Bouveret

   Article

   Gully et al Proteomics. 2006 Jan . 6(1):282-93.

   Type

   Empty backbone

   Use

   Tags

   CBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMC00024 (aka pCW-Cas9n-Blast)

   Plasmid

   #195315

   Purpose

   Dox inducible lentiviral Cas9 nickase

   Depositor

   Raj Chari

   Insert

   Cas9-nickase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9CR5

   Plasmid

   #120227

   Purpose

   pCas9CR4 with stop codon to prevent translation of ssrA degradation tag

   Depositor

   Christopher Reisch

   Insert

   SpCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Stop codon inserted before ssrA degradation tag

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK505

   Plasmid

   #71594

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, additiona…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK506

   Plasmid

   #71596

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-165 to phenylalanine, addition…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cerulean

   Plasmid

   #15214

   Depositor

   Dave Piston

   Article

   Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

   Insert

   monomeric Cerulean
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K

   Promoter

   Available since

   June 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBCPB+

   Plasmid

   #18940

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   attP, lacZ, attB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mek2

    Plasmid

    #40776

    Depositor

    Dustin Maly

    Insert

    MKK2 (MAP2K2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only