We narrowed to 7,812 results for: sms

1. FLAG-FullLength-TET1-CXXC(24)

   Plasmid

   #124398

   Purpose

   Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   FLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CXXC zinc finger deletion (aa 585-624)

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-FoxO1_1R_13A_3D

   Plasmid

   #106279

   Purpose

   Fluorescent fusion protein for FoxO1

   Depositor

   Laura Heiser

   Article

   Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

   Inserts

   Foxo1a (Foxo1 Mouse)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Tags

   Clover fluorescent protein and NLS

   Expression

   Mammalian

   Mutation

   Deleted amino acids 401-636, T24A, S209A, H212R, …

   Promoter

   EF1a/RPBSA

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

   Plasmid

   #61411

   Purpose

   Expresses human OTUD3 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 276-398.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINK-OTUD5 (OTU, aa 171-358)

   Plasmid

   #61415

   Purpose

   Expresses human OTUD5 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD5 (OTUD5 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-170 and aa 359-571.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVL1393-MAL-SYK

   Plasmid

   #157990

   Purpose

   Expresses SYK in insect cell (sf9)

   Depositor

   Byung Il Lee

   Article

   Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

   Insert

   Spleen tyrosine kinase (SYK Human)
   

   Tags

   Met-Ala-Leu and add Leu,Glu residues +His tag

   Expression

   Insect

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

   Plasmid

   #132686

   Purpose

   AAV plasmid encoding mouse ACY1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Acylase I (Acy1, Acy1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   Chicken b-actin

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. STK24_HUMAN_D0

   Plasmid

   #79742

   Purpose

   This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK24 (STK24 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pRepair-mScI-CTNNB1

   Plasmid

   #153431

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   mScarlet-i

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LENTICRISPR-HK2_sgRNA1

   Plasmid

   #201596

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPI-LUC7L-3xFLAG

    Plasmid

    #201640

    Purpose

    Over-expression of 3xFLAG-tagged human LUC7L

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L (LUC7L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPM-miR30-EIF2AK2-ts1

    Plasmid

    #174247

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFP-Parkin C431N

    Plasmid

    #46924

    Purpose

    expresses mutant Parkin with YFP tag in mammalian cells

    Depositor

    Richard Youle

    Article

    Lazarou et al J Cell Biol. 2013 Jan 21;200(2):163-72.

    Insert

    Park2 (PRKN Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed cysteine 431 to asparagine

    Promoter

    CMV

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p3s-ABE7.10

    Plasmid

    #113128

    Purpose

    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE 7.10
    

    Tags

    HA epitope and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-mCherry-LacR-BRCA1 1366-1437

    Plasmid

    #71118

    Purpose

    mammalian expession of mCherry-LacR-BRCA1 1366-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains amino acids 1366-1437 of BRCA1

    Promoter

    CMV/TO

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPU-huMDA5-miR30-MDA5

    Plasmid

    #174224

    Purpose

    All-in-one huMDA5 rescue-MDA5 shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MDA5 (IFIH1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171595

    Purpose

    Weak human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET48 AtGUN1-SMR1

    Plasmid

    #136361

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
    

    Tags

    TRX-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET48 AtGUN1-SMR2

    Plasmid

    #136362

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
    

    Tags

    TRX-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET48 AtGUN1-PPR1

    Plasmid

    #136359

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
    

    Tags

    TRX-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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