We narrowed to 7,746 results for: sms

1. pROF402

   Plasmid

   #155334

   Purpose

   Binary vector for expression of FLuc controlled by the blue light-repressible system 

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
   

   Use

   Luciferase and Synthetic Biology; Eukaryotic expr…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROF441

   Plasmid

   #155336

   Purpose

   Binary vector for expression of a gRNA targeting AtAP1 promoter 

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOPINK-VCPIP (OTU, aa 25-561)

   Plasmid

   #61583

   Purpose

   Expresses human VCPIP (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   VCPIP (VCPIP1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-24 and aa 562-1222.

   Promoter

   T7

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a-SARS-CoV2-ORF3b (S23Q)

   Plasmid

   #165088

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Stop codon to glutamine at 23rd amino acid

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCX4-cMyc

   Plasmid

   #36119

   Depositor

   Steven Dowdy

   Article

   Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

   Insert

   cMyc (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pDC184

   Plasmid

   #70055

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with p045

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   His tagged Synaptobrevin 28-89
   Syntaxin 191-256
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

8. CDK2_HUMAN_D0

   Plasmid

   #79726

   Purpose

   This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pOPINK-OTUD1 (OTU, aa 287-437)

   Plasmid

   #61407

   Purpose

   Expresses human OTUD1 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286 and aa 438-481.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LENTICRISPR-HNRNPD_sgRNA1

    Plasmid

    #201598

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HNRNPD (HNRNPD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pOPINK-OTUB2 (full-length, aa 1-234)

    Plasmid

    #61421

    Purpose

    Expresses human OTUB2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRepair-SYFP2-CTNNB1

    Plasmid

    #153432

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    SYFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid

    #108484

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6P1-Ataxin3-1-182

    Plasmid

    #22115

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 183-373 deleted

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pRevTRE MKL1-N100 S449A/T450A/S454A

    Plasmid

    #19849

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1-N100 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    deleted amino acids 1-100 and changed Serine 449,…

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pRF+275Dux4

    Plasmid

    #21293

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX 4 (DUX4 Human, DUX 4)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

19. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

    Plasmid

    #205444

    Purpose

    human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-HLA-E SCT-hCD55
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LENTICRISPR-HK2_sgRNA2

    Plasmid

    #201597

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge