We narrowed to 8,752 results for: APP

1. pMCL8_28bp

   Plasmid

   #176185

   Purpose

   plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMCL9

   Plasmid

   #176186

   Purpose

   2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC8b-XI1up

   Plasmid

   #176166

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XI-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMC7-XI1dw

   Plasmid

   #176167

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMC8b-XI2up

   Plasmid

   #176168

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XI-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC7-XI2dw

   Plasmid

   #176169

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMC8b-XII2up

   Plasmid

   #176174

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XII-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMC7-XII2dw

   Plasmid

   #176175

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XII-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC8b-X4up

   Plasmid

   #176164

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site X-4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC7-X4dw

    Plasmid

    #176165

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site X-4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJL30

    Plasmid

    #174064

    Purpose

    Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176577

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S_P0526-sgRNArodA

    Plasmid

    #149658

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. acta1-mRuby2-VHH[GFP]

    Plasmid

    #163856

    Purpose

    Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    VHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT3TS-CD44b-TurboID

    Plasmid

    #163850

    Purpose

    In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    CD44b (cd44b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJL15

    Plasmid

    #174069

    Purpose

    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBluescript-Q22YU3-KO

    Plasmid

    #170313

    Purpose

    Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 knockout
    

    Use

    Tetrahymena somatic gene knockout vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBluescript-Q22MS1-KO

    Plasmid

    #170312

    Purpose

    Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1 knockout
    

    Use

    Tetrahymena somatic gene knockout vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACEBac1-Q22MS1-2xStrep

    Plasmid

    #170314

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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