We narrowed to 207,561 results for: des

1. pRSF-KpDcr1delta2d(E224Q)

   Plasmid

   #32029

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1(15-260)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   E224Q

   Promoter

   T7

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSF-KpDcr1delta2d

   Plasmid

   #32028

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1(15-260)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSF-KpDcr1deltaC(K217A)

   Plasmid

   #32026

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1(15-355)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   K217A

   Promoter

   T7

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSF-KpDcr1deltaC(N184A)

   Plasmid

   #32025

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1(15-355)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   N184A

   Promoter

   T7

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Rab27A

   Plasmid

   #89237

   Purpose

   Expression of GFP-tagged human Rab27A in mammalian cells

   Depositor

   William Gahl

   Article

   Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

   Insert

   human Rab27A (RAB7A Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DYRK1A gRNA (BRDN0001145031)

   Plasmid

   #76755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DYRK1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-Myc-AIM2

   Plasmid

   #73958

   Purpose

   Express c-Myc-tagged human AIM2 in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

   Insert

   absent in melanoma 2 (AIM2) (AIM2 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV iE

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eGFPC1 FKBP

   Plasmid

   #67529

   Purpose

   Expresses GFP fused FKBP

   Depositor

   Stephen Tait

   Article

   Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

   Insert

   FKBP
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   F36V

   Promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pHLmMBP-10

    Plasmid

    #72348

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX_TRC311 ETV4-L

    Plasmid

    #74982

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S396N

    Promoter

    EF1a

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. VN-SOD1

    Plasmid

    #89757

    Purpose

    expresses human SOD1 fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58694

    Purpose

    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Csplit Intein and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TBX5-FLAG in pLX303

    Plasmid

    #42563

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAVS1-PC-GCaMP6f

    Plasmid

    #73503

    Purpose

    GCaMP6f knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SOD1-VC

    Plasmid

    #89758

    Purpose

    expresses human SOD1 fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX304-YAP1(S94A)

    Plasmid

    #59145

    Purpose

    YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S94A

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. shTBX5 # 2

    Plasmid

    #42550

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

22. shTBX5 # 1

    Plasmid

    #42549

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pLX304-FOS-V5

    Plasmid

    #59140

    Purpose

    wild-type FOS with C-terminal V5 tag expressed from pLX vector

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    FOS (FOS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FXN gRNA (BRDN0001144784)

    Plasmid

    #77813

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FXN

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FXN (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FXN gRNA (BRDN0001145648)

    Plasmid

    #77814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FXN

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FXN (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FXN gRNA (BRDN0001146360)

    Plasmid

    #77815

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FXN

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FXN (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiGuide-Crimson

    Plasmid

    #70683

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    S. pyogenes sgRNA cassette
    Crimson Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MigR1-AE

    Plasmid

    #12431

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DGKQ gRNA (BRDN0001162275)

    Plasmid

    #77377

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKQ

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKQ (DGKQ Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAP3K3 gRNA (BRDN0001148097)

    Plasmid

    #76016

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K3 (MAP3K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SRC gRNA (BRDN0001145402)

    Plasmid

    #75650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SRC gRNA (BRDN0001149457)

    Plasmid

    #75652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SRC gRNA (BRDN0001146185)

    Plasmid

    #75651

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CDK16 gRNA (BRDN0001147125)

    Plasmid

    #76903

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CDK16 gRNA (BRDN0001146694)

    Plasmid

    #76901

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CDK16 gRNA (BRDN0001147123)

    Plasmid

    #76902

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MASTL gRNA (BRDN0001148591)

    Plasmid

    #75989

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MASTL (MASTL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiCRISPR - EGFP sgRNA 2

    Plasmid

    #51761

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCag

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LRPPRC gRNA (BRDN0001145987)

    Plasmid

    #77773

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LRPPRC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LRPPRC (LRPPRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge