We narrowed to 10,314 results for: Rras

1. pBzCas13b-gRNA-NT

   Plasmid

   #164861

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-NT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBzCas13b-gRNA-1

   Plasmid

   #164858

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5 HA-RagC(L91P)

   Plasmid

   #99721

   Purpose

   Mammalian expression of HA-RagC(L91P)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   RagC (RRAGC )
   

   Use

   Tags

   HA

   Expression

   Mutation

   L91P

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5 HA-RagA(R37P)

   Plasmid

   #99713

   Purpose

   Mammalian expression of HA-RagA(R37P)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   RagA (RRAGA )
   

   Use

   Tags

   HA

   Expression

   Mutation

   R37P

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFUS2_b4

   Plasmid

   #50698

   Purpose

   Intermediate array vector

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   LacZ + BsaI restriction sites
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFUS2_b1

   Plasmid

   #50695

   Purpose

   Intermediate array vector

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   LacZ + BsaI restriction sites
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFUS2_a2a

   Plasmid

   #50681

   Purpose

   Intermediate array vector

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   LacZ + BsaI restriction sites
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFUS2_a2b

   Plasmid

   #50682

   Purpose

   Intermediate array vector

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   LacZ + BsaI restriction sites
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX Rad

   Plasmid

   #11227

   Depositor

   Ronald Kahn

   Article

   Zhu et al J Biol Chem. 1995 Mar 3. 270(9):4805-12.

   Insert

   Rad (RRAD Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N39: deletion of amino acids 1-39

   Promoter

   Available since

   March 1, 2006

   Availability

   Academic Institutions and Nonprofits only

10. MCHR1B_R (TAL 3009)

    Plasmid

    #36001

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting MCHR1B (mchr1b Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MCHR1B_L (TAL 3008)

    Plasmid

    #36000

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting MCHR1B (mchr1b Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE Rad C249

    Plasmid

    #11215

    Depositor

    Ronald Kahn

    Article

    Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

    Insert

    Rad (RRAD Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C249: deletion of amino acids 248-308

    Promoter

    Available since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

13. GDF2_R (TAL 3011)

    Plasmid

    #36003

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting GDF2 (gdf2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GDF2_L (TAL 3010)

    Plasmid

    #36002

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting GDF2 (gdf2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p140Tol2-Olactb:SABER

    Plasmid

    #226829

    Purpose

    Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    BFP-SABER
    mCherry-t2A-CreERT2
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Olactb and hsp70

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.26_4xUAS_DSCP_GFP

    Plasmid

    #111931

    Purpose

    Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

    Depositor

    Alexander Stark

    Article

    Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

    Insert

    4xUAS GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTAG4E

    Plasmid

    #176868

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_4E
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTAG5

    Plasmid

    #176869

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAG6E

    Plasmid

    #176870

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_6E
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTAG2

    Plasmid

    #176864

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTAG3

    Plasmid

    #176866

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTAG4

    Plasmid

    #176867

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJL50

    Plasmid

    #174068

    Purpose

    Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pTEF1-3NLS-97-3ZF-VP16
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBzCas13b-gRNA-2

    Plasmid

    #164859

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBzCas13b-gRNA-3

    Plasmid

    #164860

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLsCas13a-gRNA-3

    Plasmid

    #164867

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLsCas13a-gRNA-2

    Plasmid

    #164866

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAS2-1-Kre33

    Plasmid

    #99989

    Purpose

    Express yeast Kre33 with Y2H binding domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Kre33 (KRE33 Budding Yeast)
    

    Use

    Tags

    Y2H binding domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

30. pFUS2_a3a

    Plasmid

    #50683

    Purpose

    Intermediate array vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    LacZ + BsaI restriction sites
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFUS2_a3b

    Plasmid

    #50684

    Purpose

    Intermediate array vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    LacZ + BsaI restriction sites
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFUS2_a4a

    Plasmid

    #50685

    Purpose

    Intermediate array vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    LacZ + BsaI restriction sites
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFUS2_a4b

    Plasmid

    #50686

    Purpose

    Intermediate array vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    LacZ + BsaI restriction sites
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFUS2_b3

    Plasmid

    #50697

    Purpose

    Intermediate array vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    LacZ + BsaI restriction sites
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFUS2_b2

    Plasmid

    #50696

    Purpose

    Intermediate array vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    LacZ + BsaI restriction sites
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Zinc Finger Consortium Expression Vector and Modular Assembly Kit

    Plasmid Kit

    #1000000005

    Purpose

    Can be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.

    Depositor

    Keith Joung

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pFREE

    Plasmid

    #92050

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDGE508

    Plasmid

    #153280

    Purpose

    End-linker for cloning 2 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFREE_Zeo

    Plasmid

    #92053

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSIMcpf1

    Plasmid

    #153034

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. HP138-neo

    Plasmid

    #134247

    Purpose

    Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    Contains Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

    Plasmid

    #159899

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

    Plasmid

    #173230

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

    Plasmid

    #173231

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. PAmCherry1-C1-P4Mx2

    Plasmid

    #202681

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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