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pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

Plasmid

#176535

Purpose

Non-protein coding locus components from CRISPR-associated IscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

T301N/N323S

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

Plasmid

#174336

Purpose

The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Retroviral

Tags

pHluorin inserted in the extracellular loop 2 of …

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_fibrillarin

Plasmid

#65709

Purpose

Expresses CNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
gRNAs[bTub+Sxl].1046B

Plasmid

#112692

Purpose

express two gRNA targeting bTub & Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a-Q604L

Plasmid

#79595

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Q604L

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-T559N

Plasmid

#79594

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

T559N

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pCR2.1-INS-904

Plasmid

#53970

Purpose

904 bp insert from human INS gene used as a control for methylation-specific PCR assays

Depositor

Kevin Ferreri

Article

Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

Insert

human INS gene partial (INS Human)

Expression

Bacterial

Promoter

lac

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMSL88

Plasmid

#254505

Purpose

Expresses Type IV-A1 Cas5,6,7,8, Repeat-Spacer-Repeat CRISPR-RNA, and CasDinG-HNH from Pseudomonas oleovorans. CasDinG was engineered to include a C-terminal HNH nuclease domain

Depositor

Lennart Randau

Article

Klein et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Inserts

Cas6
Cas8
Cas7
Cas5
CRISPR-RNA
CasDinG-HNH

Tags

HNH domain

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1085 pET His-Sumo-ORF

Plasmid

#250758

Purpose

Vector for bacterial expression of proteins with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Integrin-b3-Halo

Plasmid

#249172

Purpose

Mammalian expression vector of human ITGB3 fused with Halo-tag

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Integrin beta-3 (ITGB3 Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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GFP-CDKL5(isoform-2)

Plasmid

#245901

Purpose

Mammalian expression of CDKL5 isoform2 (1-1030) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-1 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-CDKL5(kinase)

Plasmid

#245902

Purpose

Mammalian expression of CDKL5 kinase domain (1-311) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CDKL5(isoform1)-GFP

Plasmid

#245903

Purpose

Mammalian expression of CDKL5 isoform1 (1-960) with C-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGBKT7_AvrSr62-1

Plasmid

#233531

Purpose

Expresses AvrSr62-1 in yeast

Depositor

Brande Wulff

Article

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

AvrSr62-1

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR137

Plasmid

#226809

Purpose

Gateway entry vector for GPR137

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSN554

Plasmid

#177361

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

Leucine zipper, StrepII tag, and mScarlet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-HOXD13(+10A)-IDR

Plasmid

#145257

Purpose

Expresses fusion of ECFP-LacI and HOXD13+10A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+10A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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