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pcDNA3.1-HA-GTSE1-S91A

Plasmid

#224721

Purpose

For expression of HA-tagged GTSE1 protein with S91A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 91 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S157A

Plasmid

#224722

Purpose

For expression of HA-tagged GTSE1 protein with S157A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 157 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8

Plasmid

#224208

Purpose

Expresses human KDAC8 (HDAC8) in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Promoter

T5

Available Since

Dec. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Di-iPGM

Plasmid

#180241

Purpose

Bacterial expression plasmid for production of recombinant Dirofilaria immitis iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Dirofilaria immitis iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4

Plasmid

#200230

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaVB2

Plasmid

#200231

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV beta 2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.5

Plasmid

#200232

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis Nav1.5

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N432T

Plasmid

#200284

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N432T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N830T

Plasmid

#200285

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N830T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N11306T

Plasmid

#200286

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N11306T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N1609T

Plasmid

#200287

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N1609T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N1609A

Plasmid

#200288

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N1609A

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pt NaV1.4-N1600T

Plasmid

#200289

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Expression

Mammalian

Mutation

N1600T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Dt NaV1.4-N1600T

Plasmid

#200308

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Dendrobates tinctorius NaV1.4 – muscle channel

Expression

Mammalian

Mutation

N1600T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-NOT1 isoform C-mCherry-PH (EB5)

Plasmid

#206474

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mcherry PH

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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