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2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-TrxC

Plasmid

#225742

Purpose

To express TrxC(Rv3914) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxC

Tags

6xHis

Expression

Bacterial

Mutation

TrxC

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-TrxB2

Plasmid

#225741

Purpose

To express TrxB2(Rv3913) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxB2

Tags

6xHis

Expression

Bacterial

Mutation

TrxB2

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelI

Plasmid

#225730

Purpose

To express MelI (Rv1939) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melI

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelK

Plasmid

#225732

Purpose

To express MelK (Rv1941) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melK

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelJ

Plasmid

#225731

Purpose

To express MelJ (Rv1940) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melJ

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89055

Purpose

PV2 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89053

Purpose

PV1 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA

Plasmid

#87576

Purpose

PV3 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA

Plasmid

#87582

Purpose

PV3 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW807_pCAG-PV4-loxP-mCherry-BGHpA

Plasmid

#87583

Purpose

PV4 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

Plasmid

#87577

Purpose

PV4 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW707_pCAG-PV3-FRT-loxP-mCherry

Plasmid

#87571

Purpose

PV3 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW705_pCAG-PV1-FRT-lox2272-STOP

Plasmid

#87569

Purpose

PV1 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW706_pCAG-PV2-lox2272-GFP

Plasmid

#87570

Purpose

PV2 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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