We narrowed to 4,907 results for: gast

1. pMAL c2x Tral N

   Plasmid

   #113009

   Purpose

   For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   N terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 1-355

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL c2x Tral FL-15A

   Plasmid

   #113011

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to A

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL c2x Tral C

   Plasmid

   #113008

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 356-652

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL c2x Tral C 15A

   Plasmid

   #113007

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PNG phosphorylation sites mutated to A

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFOSGE_COF4D03

   Plasmid

   #70969

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Taf12 (Taf12 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pFOSGE_CUS1F03

   Plasmid

   #71071

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Su(var)205 (Su(var)205 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. KHBD00295

   Plasmid

   #34421

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG12052 (lola Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

8. KHBD00321

   Plasmid

   #34446

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG8676 (Hr39 Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. KHBD00050

   Plasmid

   #34212

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG11502 (svp Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

10. KHBD00060

    Plasmid

    #34221

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9415 (Xbp1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. KHBD00012

    Plasmid

    #34174

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8328 (HLHmdelta Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. BII-CMV-AfmW3A

    Plasmid

    #133402

    Purpose

    Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Afamin and Wnt3a (Afm, Wnt3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Mutation

    Promoter

    osk

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159676

    Purpose

    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

    Plasmid

    #211817

    Purpose

    AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    N-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter and U6 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

    Plasmid

    #211818

    Purpose

    AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    C-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter and U6 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BII-ChBtW-dnTCF4-ires-GFP

    Plasmid

    #133378

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    dnTCF4 (TCF4 Human)
    

    Use

    Tags

    2x Flag

    Expression

    Mammalian

    Mutation

    deltaN31

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT-EGFP-Actin5c

    Plasmid

    #15312

    Depositor

    Ron Vale

    Article

    Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

    Insert

    Actin5c (Act5C Fly)
    

    Use

    Tags

    EGFP and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only