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pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L

Plasmid

#146861

Purpose

Insect Expression of DmPABPC1_del91-164

Depositor

Elisa Izaurralde

Publication

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del91-164 (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral_99-393-V5His6_D

Plasmid

#146137

Purpose

Insect Expression of DmTral_99-393

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_99-393 (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral_394-543-V5His6_D

Plasmid

#146138

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-DFDF-KFKF_C

Plasmid

#146038

Purpose

Insect Expression of DmTral-DFDF-KFKF

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-DFDF-KFKF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

Plasmid

#146071

Purpose

Insect Expression of DmTral-LSmR21EE23KR42EF44A

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KR42EF44A (tral Fly)

Expression

Insect

Mutation

R21E, E23K, R42E, F44A. Additionally, one silent …

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

Plasmid

#146049

Purpose

Insect Expression of DmTral-LSmR21EE23KS13AI15A

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KS13AI15A (tral Fly)

Expression

Insect

Mutation

S13A, I15A, R21E, E23K, one silent mutation A198C…

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00053

Plasmid

#34215

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T

Plasmid

#147592

Purpose

Insect Expression of DmThor-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Publication

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmeIF4Ga_687-1666 (Thor Fly)

Expression

Insect

Mutation

DmThor two silent mutations A246C and T321C compa…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-861_R

Plasmid

#147404

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-861

Depositor

Elisa Izaurralde

Publication

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-861 (gw Nematode, Fly)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-1384_R

Plasmid

#147405

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384

Depositor

Elisa Izaurralde

Publication

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384 (gw Nematode, Fly)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L

Plasmid

#146862

Purpose

Insect Expression of DmPABPC1_del183-258-siRNAres

Depositor

Elisa Izaurralde

Publication

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del183-258-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L

Plasmid

#146863

Purpose

Insect Expression of DmPABPC1_del288-359-siRNAres

Depositor

Elisa Izaurralde

Publication

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del288-359-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and six silent muta…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L

Plasmid

#146864

Purpose

Insect Expression of DmPABPC1_del364-550-siRNAres

Depositor

Elisa Izaurralde

Publication

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del364-550-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Publication

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Andrew Allen, Ross Bathgate

Publication

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

CMV promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHD-dsRed-attP_dCrk_flankingHRs

Plasmid

#131141

Purpose

Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

Depositor

Mark Peifer

Publication

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Inserts

Crk Upstream Homology Region (Crk Fly)
Crk Downstream Homology Region (Crk Fly)

Use

CRISPR and Cre/Lox; Homology directed repair temp…

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D03

Plasmid

#70969

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12 (Taf12 Fly)

Use

Gateway Cloning

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway Cloning

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00450

Plasmid

#39563

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway Cloning

Promoter

none

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only