We narrowed to 13,743 results for: TIM;

1. pMY-eGFP-P2A-PuroR-HA

   Plasmid

   #163343

   Purpose

   Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cre-responsive Cas9 expressing transposon library vector

   Plasmid

   #164138

   Purpose

   Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6, EF-1a

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(DAP10)

   Plasmid

   #177904

   Purpose

   Bacterial expression of his10-tagged human DAP10 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   DAP10 (aa 70-92 only) (HCST Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-mir144 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164791

   Purpose

   Express miR-144 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144 (MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19.TRIM28.FKBP.knock-in.mCerulean

   Plasmid

   #185009

   Purpose

   For knocking in FKBP F36V P2A mCerulean

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   FKBP F36V P2A mCerulean
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn1 ChR2 ET-TC 2A tDimer

   Plasmid

   #101361

   Purpose

   High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   synaptophysin-red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   human Synapsin

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRN3P-HA-Suv4-20h1

   Plasmid

   #86689

   Purpose

   For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mutation

   Promoter

   T3

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MSP1D1deltaH4/2H5

   Plasmid

   #71715

   Purpose

   Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMY-mCherry-P2A-PuroR-HA

    Plasmid

    #163342

    Purpose

    Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-FOXO3a T32A DBM (H212R)

    Plasmid

    #1793

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, H212R

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pBI-CMV1-mir451~144-CMV2-mir1

    Plasmid

    #164666

    Purpose

    Express miR-451~144 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-451~144 (MIR451A Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid

    #115137

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2.1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-MCU-S259A

    Plasmid

    #31728

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Changed S259 to Alanine

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

16. (401) pAAV CMV-ZsGreen U6-gRNA

    Plasmid

    #163021

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQZ210

    Plasmid

    #106414

    Purpose

    Co-expresses trans SNARE complex with pDC183 in E.coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Inserts

    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Fragment 191-256 , Codon optimization and Fragmen…

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA-SCW-HHL

    Plasmid

    #190634

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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