We narrowed to 13,705 results for: gan

1. pTetOne-FLAG-human cGASdeltaN-HA

   Plasmid

   #186899

   Purpose

   Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Mutation

   N-terminal truncation

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

   Plasmid

   #221329

   Purpose

   Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)
   

   Use

   Retroviral

   Mutation

   G12V

   Promoter

   TRE3G

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcdna6.2- myc ULK1 k46I

   Plasmid

   #27630

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Available Since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pcdna6.2- myc ULK1 4SA

   Plasmid

   #27631

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 4SA (Myc Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S467A, S555A, T574A, S637A. Initial M from ULK1 r…

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIN- myc ULK1 wt

   Plasmid

   #27626

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 (Myc Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIN- myc ULK1 4SA

   Plasmid

   #27628

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 4SA (Myc Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Mutation

   S467A, S555A, T574A, S637A. Initial M from ULK1 r…

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pENTR1A-Yap S127A

   Plasmid

   #46050

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Entry vector

   Tags

   2X Flag tag

   Mutation

   S127A

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-FLAG_FBXL7 (R310H)

   Plasmid

   #171849

   Purpose

   pcDNA3-FLAG_FBXL7 (R310H)

   Depositor

   Michele Pagano

   Article

   Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

   Insert

   F-box and leucine rich repeat protein 7 (FBXL7 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Arginine 310 to Histidine

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCXIN- myc ULK1 K46I

   Plasmid

   #27627

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 ATP5O(TISU)-FF

    Plasmid

    #85489

    Purpose

    Firefly luciferase under the control of ATP5O TISU

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    Full TISU element of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    2nd and 3rd codon of luciferase were modified fro…

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 - myc ULK1 k46I

    Plasmid

    #27624

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pDONR221 - myc ULK1 4SA

    Plasmid

    #27625

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

14. AAV-FLEX-CAG_G-PTEN

    Plasmid

    #227438

    Purpose

    An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

    Depositor

    Tal Laviv

    Article

    Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

    Insert

    G-PTEN sensor (Pten Rat)
    

    Use

    AAV

    Tags

    CD-sREACh and mEGFP

    Expression

    Mammalian

    Mutation

    R14G

    Promoter

    pCAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 FFSS-BACH1 C122E

    Plasmid

    #232274

    Purpose

    Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    changed cysteine 122 to glutamic acid

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVtrunc LifeactΔC4-msfGFPΔN7

    Plasmid

    #231555

    Purpose

    Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227925

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227926

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227911

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227914

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge