We narrowed to 152,943 results for: des
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pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9_sgAAVS1
Plasmid#199213PurposeAll-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.ÂDepositorInsertAAVS1-gRNA
UseCRISPRTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
LCV2 STING KO
Plasmid#217445PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human STINGDepositorInsertsgRNA targeting STING (STING1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-L
Plasmid#74982PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorInsertETV4 (ETV4 Human)
UseTagsExpressionMammalianMutationS396NPromoterEF1aAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDP082
Plasmid#103875PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianusDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-GFP
Plasmid#115495PurposeLentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)DepositorInserteGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL-UCOE-SFFV-TLR-MCV1
Plasmid#117111PurposeLentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.DepositorInsertUCOE SFFV Traffic Light Reporter 2.0
UseLentiviral; ReporterTagsExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Stx5L-mCherry
Plasmid#209844PurposeExpresses Stx5L-mCherry in mammalian cellsDepositorInsertSTX5 (STX5 Human)
UseTagsmCherryExpressionMammalianMutationWildtype Stx5 (with 55M)PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
IQGAP1_pLX307
Plasmid#98346PurposeLentiviral expression of IQGAP1DepositorInsertIQGAP1 (IQGAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-HA-GOLPH3
Plasmid#21687DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASH
Plasmid#125554PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationTruncated form: only the KASH domain of nesprin-…Promotertetracycline responsive elementAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX317-SNAI1
Plasmid#115446PurposeConstitutive lentiviral expressionDepositorInsertSNAI1 (SNAI1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCas9-Geminin
Plasmid#109401PurposeExpresses Cas9 fusion to Geminin degron domainDepositorInsertGeminin (GMNN Human)
UseTagsExpressionMutationGeminin degron fragmentPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
C-Terminal Split Cas9 with GyrA intein
Plasmid#58694PurposeExpresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
UseAAV and CRISPRTagsGyrA Csplit Intein and NLSExpressionMammalianMutationPromoterCBhAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MYC_pLX307
Plasmid#98363PurposeLentiviral expression of MYCDepositorInsertMYC (MYC Human)
UseLentiviralTagsNAExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TBX5-FLAG in pLX303
Plasmid#42563DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
LCV2 cGAS KO
Plasmid#217444PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human cGASDepositorInsertsgRNA targeting cGAS (CGAS Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PC-GCaMP6f
Plasmid#73503PurposeGCaMP6f knock in construct into the AAVS1 locusDepositorInsertGCaMP6f
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pLX304-YAP1(S94A)
Plasmid#59145PurposeYAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factorsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationS94APromoterCMVAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianMutationPromoterCBhAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
shTBX5 # 2
Plasmid#42550DepositorInsertshTBX5 (TBX5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
shTBX5 # 1
Plasmid#42549DepositorInsertshTBX5 (TBX5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB-Gaolf-Integration
Plasmid#129456PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses GaolfDepositorInsertG alpha olf (Gnal Mouse)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE (Tet-On)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9-HE
Plasmid#109400PurposeExpresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP proteinDepositorInsertHDR-Enhancer domain of human CtIP protein (RBBP8 Human)
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP MASTL WT (siRNA resistant)
Plasmid#191011PurposeExpresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Myc2- Hs TTP WT
Plasmid#107008PurposeExpresses epitope-tagged wild-type human TTPDepositorInserttristetraprolin (ZFP36 Human)
UseTagsDouble Myc peptide epitope tagExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP MASTL G44S (siRNA resistant)
Plasmid#191012PurposeExpresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationG44SPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304-FOS-V5
Plasmid#59140Purposewild-type FOS with C-terminal V5 tag expressed from pLX vectorDepositorInsertFOS (FOS Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
FXN gRNA (BRDN0001144784)
Plasmid#77813Purpose3rd generation lentiviral gRNA plasmid targeting human FXNDepositorInsertFXN (FXN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FXN gRNA (BRDN0001145648)
Plasmid#77814Purpose3rd generation lentiviral gRNA plasmid targeting human FXNDepositorInsertFXN (FXN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FXN gRNA (BRDN0001146360)
Plasmid#77815Purpose3rd generation lentiviral gRNA plasmid targeting human FXNDepositorInsertFXN (FXN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-FL
Plasmid#105670PurposeMammalian expression construct of mouse SIRT1 full length isoformDepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-WT
Plasmid#114044PurposeExpression of exogenous LAP tagged MPS1 WTDepositorInsertMPS1-WT (TTK Human)
UseTagsExpressionMammalianMutationWT MPS1 sequencePromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-dnTCF4
Plasmid#114277PurposeTET-inducible expression of a dominant negative form of the TCF4 transcription factorDepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMutationdeletion of AA 1 to 31PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only
