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pAGM9121_TEV-His-GFP11

Plasmid

#153510

Purpose

TEV-His-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TEV-His-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Acid Phosphatase SP

Plasmid

#153494

Purpose

S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Acid Phosphatase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Gma-UPO SP

Plasmid

#153495

Purpose

Galerina marginata Unspecific Peroxygenase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Unspecific peroxygenase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H(-10TC)_arr2

Plasmid

#149587

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAflaB

Plasmid

#149561

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

Plasmid

#162479

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

Plasmid

#162474

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

Plasmid

#162480

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

DNE-Frterminator

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PflaBS-sgRNAflaB

Plasmid

#149643

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PflaBS

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_arr2

Plasmid

#149582

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNArodA

Plasmid

#149569

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826L-sgRNAflaB

Plasmid

#149648

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826L

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNArodA

Plasmid

#149655

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTS-sgRNAflaB

Plasmid

#149644

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTL-sgRNAflaB

Plasmid

#149645

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTL

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0026-sgRNAflaB

Plasmid

#149646

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826S-sgRNAflaB

Plasmid

#149647

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826S

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAftsI

Plasmid

#149649

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAftsI

Plasmid

#149650

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G(-10TC)_arr2

Plasmid

#149585

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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