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pHH0103_ITSN1-1/5-IS2/3

Plasmid

#91318

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS2/3 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1-1/1-SV2/2

Plasmid

#91392

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV2/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MIA3-1/1

Plasmid

#91327

Purpose

Protein expression and purification of human SH3 domain construct MIA3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MIA3-1/1 (MIA3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLCG1-1/1

Plasmid

#91540

Purpose

Protein expression and purification of human SH3 domain construct PLCG1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PLCG1-1/1 (PLCG1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

Plasmid

#73306

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

Q867E-Q939E-C935S-Q1011E-C1007S

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R514S-YFP

Plasmid

#29617

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R514S

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P27A)

Plasmid

#62068

Purpose

Expression of human p53 (1-73) (P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P27A

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R524S-YFP

Plasmid

#29626

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R524S

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R522G-YFP

Plasmid

#29625

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R522G

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
SIN-cPPT-G1B3-mitoGO-ATeam2-WPRE-miR124T

Plasmid

#245075

Purpose

Mitochondrial ATP biosensor, with miRNA-124 target sequence to repress transgene expression in neurons

Depositor

Nicole Déglon

Article

Richetin et al Nat Neurosci. 2020 Dec;23(12):1567-1579.

Insert

mitoGO-ATeam2

Use

Lentiviral

Expression

Mammalian

Promoter

Human G1B3 promoter

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLOC-D133p53a

Plasmid

#241916

Purpose

Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.

Depositor

Curtis Harris

Article

Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. d

Insert

Delta133p53alpha (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX RBM42-HA

Plasmid

#229500

Purpose

Lentiviral expression vector to express RBM42 isoform 1 with HA-tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

RBM42 (RBM42 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_SH3(W765A)_GFP

Plasmid

#222616

Purpose

Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation W765A (SH3 domain)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_SH3(W765A)_HA

Plasmid

#222614

Purpose

Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

HA

Expression

Mammalian

Mutation

Point mutation W765A (SH3 domain)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG SRGAP2A_EVH1(P339L F342C)

Plasmid

#222613

Purpose

Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Point mutations P339L and F342C

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXB1-WT

Plasmid

#215589

Purpose

For overexpression of Gal4-DBD HOXB1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXB1 (HOXB1 Human)

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_YtoW

Plasmid

#224253

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_YtoW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_YtoW (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y52W, Y59W, Y75W, Y81W, Y110W, Y120W, Y129W

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa_WT(N)

Plasmid

#215635

Purpose

For overexpression of Gal4-DBD CEBPa WT(N)

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa(N) (CEBPA Human)

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG-LRRC37B

Plasmid

#220790

Purpose

To express the human receptor LRRC37B in mammalian cells together with EGFP

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

Insert

LRRC37B (LRRC37B Human)

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat DJ-1 shRNA

Plasmid

#222870

Purpose

Expresses mTagBFP2 along with an shRNA against rat DJ-1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 shRNA (Park7 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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