We narrowed to 4,454 results for: TIL

1. pLenti-PGK-RAP2A-V12-neo

   Plasmid

   #156178

   Purpose

   Lentiviral vector for expression of RAP2A-V12, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-flag-RAP1B-V12-neo

   Plasmid

   #156175

   Purpose

   Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-flag-RAP1B-N17-neo

   Plasmid

   #156176

   Purpose

   Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. BE3(E63Q)-P2A-EGFP (pRZ189)

   Plasmid

   #123613

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(E63Q)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E63Q in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-RAP2A-N17-neo

   Plasmid

   #156179

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

   Plasmid

   #105803

   Purpose

   Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-FLAG; ORF2: TEV-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

   Plasmid

   #113162

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

   Plasmid

   #136659

   Purpose

   Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:TH1477 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCMVblast_SLC38A2_N82A

    Plasmid

    #156181

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TOPO-EXOC3 (-2455 to -1951)

    Plasmid

    #226441

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -2455 to -1951) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-2455 to -1951) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TOPO-EXOC3 (-1592 to -1046)

    Plasmid

    #226444

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1592 to -1046) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1592 to -1046) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TOPO-EXOC3 (-1591 to -1009)

    Plasmid

    #226443

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1591 to -1009) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1591 to -1009) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TOPO-EXOC3 (-1701 to -1594)

    Plasmid

    #226442

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1701 to -1594) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1701 to -1594) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TOPO-EXOC3 (-1592 to -1444)

    Plasmid

    #226445

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1592 to -1444) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1592 to -1444) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TOPO-EXOC3 (-1557 to-1444)

    Plasmid

    #226447

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1557 to-1444) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1557 to-1444) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IL2-AHDC2-Cherry in pmCherryN1

    Plasmid

    #187291

    Purpose

    Express pmCherry-IL2-AH-deltaC2

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AH-deltaC2

    Promoter

    CMV

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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