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pDGE14

Plasmid

#79473

Purpose

sgRNA shuttle vector; module 7

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE15

Plasmid

#79474

Purpose

sgRNA shuttle vector; module 8E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE10

Plasmid

#79466

Purpose

sgRNA shuttle vector; module 4

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pPD385 VPR-ZF43(RAAA)

Plasmid

#138824

Purpose

Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-FRB-HA no-pA

Plasmid

#82594

Purpose

3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_A4110

Plasmid

#91056

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-dTaCas9-VPR

Use

CRISPR

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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MTK0_043

Plasmid

#123943

Purpose

Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - A1 Insulator - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A1210

Plasmid

#91033

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSW177 - pCASP1

Plasmid

#115986

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Alexis Maizel

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCASP1

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#197699

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

CmYLCV promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-RLuc-9

Plasmid

#197739

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Renilla luciferase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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MTK2_008

Plasmid

#123703

Purpose

Encodes human pUbc as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phUbC

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25K

Plasmid

#91147

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TALEN

Use

TALEN

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFH51

Plasmid

#128213

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE5

Plasmid

#79461

Purpose

sgRNA shuttle vector; module 1

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK206

Plasmid

#110588

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMC-1-CmYLCV-4

Plasmid

#197694

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

CmYLCV promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLBC0010

Plasmid

#172774

Purpose

Level 0 module containing NT1_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0011

Plasmid

#172775

Purpose

Level 0 module containing NT2_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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