We narrowed to 9,297 results for: yeast expression

1. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TDH3

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT9-Hook2

   Plasmid

   #198524

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook2 (HOOK2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   His488Gln substitution

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUDR211

   Plasmid

   #113870

   Purpose

   expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

   Depositor

   Jean-Marc Daran, Jack Pronk

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA3-HXT8 / sgRNA4-HXT14
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP010

   Plasmid

   #101166

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYI2206

   Plasmid

   #228348

   Purpose

   Constitutive rPhlTA mutant expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K

   Promoter

   KpGAPDH promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT1026

   Plasmid

   #228299

   Purpose

   Constitutive expression of PhlTA mutant with weak promoter and terminator

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   E41G

   Promoter

   KpYPT1 promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT478

   Plasmid

   #228286

   Purpose

   Constitutive mutant rPhlTA expression with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   rphlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K

   Promoter

   KpGAPDH promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

   Plasmid

   #225228

   Purpose

   Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

   Inserts

   Aga2
   Htt (25Q)
   

   Use

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

   Plasmid

   #225229

   Purpose

   Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

   Inserts

   Aga2
   Htt (46Q)
   

   Use

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. BS-ZJLEU-PTEF1-GFP

    Plasmid

    #207018

    Purpose

    Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

    Plasmid

    #207024

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

    Plasmid

    #207023

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

    Plasmid

    #207022

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

    Plasmid

    #207021

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

    Plasmid

    #207020

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

    Plasmid

    #207019

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ClhN-P1K-3VSV-Atg8-MET15

    Plasmid

    #207055

    Purpose

    Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    Promoter

    pATG8

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. P1K-3HA-Atg8∆GR-MET15

    Plasmid

    #207033

    Purpose

    Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆GR

    Promoter

    pATG8

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBT9-γ1 ear

    Plasmid

    #199177

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    AP-1 γ1 ear domain (Ap1g1 Mouse)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAD424-Rabex-5

    Plasmid

    #197043

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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