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PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B

Plasmid

#250912

Purpose

Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (Arfgap1, SEC61B Rat, Human)

Tags

EGFP on Sec61B and mKate2-3xNLS on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 196–234 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-P2A-EGFP-LMNB1

Plasmid

#250905

Purpose

Dual color expression of mKate2 tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Zebrafish, Human)

Tags

EGFP on LMNB1 and mKate2 on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 192–304 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1

Plasmid

#250908

Purpose

Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-3xNLS-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Zebrafish, Human)

Tags

EGFP on LMNB1 and mKate2-3xNLS on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 192–304 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

Plasmid

#250914

Purpose

Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Zebrafish, Rat)

Tags

EGFP on LMNB1 and mKate2 on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 196–234 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPFAS-2XStrep

Plasmid

#125682

Purpose

expresses human PFAS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PFAS (PFAS Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJK531

Plasmid

#72512

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK539

Plasmid

#72515

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK549

Plasmid

#72520

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK555

Plasmid

#72521

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK577

Plasmid

#72528

Purpose

Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Use

Gene synthesis

Promoter

n/a

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK532

Plasmid

#72513

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJK544

Plasmid

#72517

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK546

Plasmid

#72518

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK567

Plasmid

#72522

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK530

Plasmid

#72511

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK533

Plasmid

#72514

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK575

Plasmid

#72526

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge