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Plasmid#73196PurposeHuman expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationR661A, Q695A, and Q926A in SpCas9PromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Mesp2
Plasmid#72693PurposeDoxycyline regulated expression of Mesp2 in mammalian cellsDepositorInsertMesp 2 (Mesp2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
VEEV_KP282671.1_NSP2_Pro_NHis_CAvi
Plasmid#234393PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_VEEV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EEEV_KP282670.1_NSP2_FL_NHis_CAvi
Plasmid#234395PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_EEEV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EEEV_KP282670.1_NSP2_Pro_NHis_CAvi
Plasmid#234396PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_EEEV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEEV_KP282671.1_NSP2_FL_NHis_CAvi
Plasmid#234392PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_VEEV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CHICKV_KY575571.1_NSP2_Pro_NHis_CAvi
Plasmid#234394PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_CHICKV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEEV_KP282671.1_NSP2_Pro_NHisTEV_CThromAvi
Plasmid#234384PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_VEEV
UseTagsLVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CHICKV_KY575571.1_NSP2_FL_NHisTEV_CThromAvi
Plasmid#234385PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_CHICKV
UseTagsLVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEEV_KP282671.1_NSP2_FL_NHisTEV_CThromAvi
Plasmid#234383PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_VEEV
UseTagsLVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
EEEV_KP282670.1_NSP2_Pro_NHisTEV_CThromAvi
Plasmid#234387PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_EEEV
UseTagsLVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CHICKV_KY575571.1_NSP2_Hel_NAviCHis
Plasmid#234397PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_CHICKV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
EEEV_KP282670.1_NSP2_Hel_NHisCAvi
Plasmid#234399PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_EEEV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEEV_KP282671.1_NSP2_Hel_NHisCAvi
Plasmid#234398PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_VEEV
UseTagsMHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CHICKV_KY575571.1_NSP2_Pro_NHisTEV_CThromAvi
Plasmid#234386PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp2_CHICKV
UseTagsLVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISVgsp2_reRNA
Plasmid#205357PurposeBacterial expression of ISVgsp2 TnpB and its reRNADepositorInsertISVgsp2 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
3SP2
Plasmid#126536Purpose3-input history dependent program 0-0-0-mKate2DepositorInsert0-0-0-mKate2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
2SP2
Plasmid#126527Purpose2-input history dependent program B then A; sfGFP-0-GFP226DepositorInsert2-input B then A; sfGFP-0-GFP226
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTDSP2_NIF
Plasmid#109831PurposeProtein expression and purification of CTDSP2_NIFDepositorInsertCTDSP2_NIF (CTDSP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only