We narrowed to 5,308 results for: pcDNA3.1+

1. pcDNA SF-HERC2 F4

   Plasmid

   #55787

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA SF-HERC2 F5

   Plasmid

   #55788

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. mKO3-TEAD4

   Plasmid

   #221217

   Purpose

   RFP fused TEAD4

   Depositor

   Xiaokun Shu

   Article

   Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

   Insert

   mKO3-TEAD4 (TEAD4 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-FlicR1

   Plasmid

   #74142

   Purpose

   Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

   Depositor

   Robert Campbell

   Article

   Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

   Insert

   FlicR1
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cytosolic FAK biosensor

   Plasmid

   #78300

   Purpose

   FRET biosensor. Visualization of cytosolic FAK activity in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Cytosolic FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICS PM-ER Short P2A

   Plasmid

   #164112

   Purpose

   Detect the short Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Lats1 (Nigg HS189)

   Plasmid

   #41156

   Depositor

   Erich Nigg

   Article

   Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

   Insert

   Lats1 (LATS1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA_RGG-GFP-RGG

   Plasmid

   #124930

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid

    #137827

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-BaLgp120-8his

    Plasmid

    #100925

    Purpose

    Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 HA Arf1 ActQ71L

    Plasmid

    #10832

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf1 Q71L (ARF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q71L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Use

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HiBiT-FZD1

    Plasmid

    #195839

    Purpose

    Expresses human FZD1 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD1 (FZD1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-noPS

    Plasmid

    #177943

    Purpose

    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA -CLCN3-3xFlag

    Plasmid

    #225506

    Purpose

    CLCN3 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    CLCN3 (CLCN3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only