We narrowed to 14,746 results for: Lif

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Dscaml1-AP-His

Plasmid

#71946

Purpose

Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscaml1 (Dscaml1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-6 [N182/17.1R]

Plasmid

#114561

Purpose

Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-QKI-6 (Human) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Islr2.b-Fc-His

Plasmid

#72077

Purpose

Expresses the extracellular region of the Islr2.b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Islr2.b-AP-His

Plasmid

#71951

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
Jam4.1-Fc-His

Plasmid

#72081

Purpose

Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.1 (Igsf5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shLRRK2-mCherry-CAAX

Plasmid

#227686

Purpose

Membrane tethered mCherry and shRNA targeting LRRK2

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

LRRK2 shRNA (Lrrk2 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shAtg7-mCherry-CAAX

Plasmid

#227687

Purpose

Membrane tethered mCherry and shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Atg7 shRNA (Atg7 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Ezrin T567A-BioID2-HA

Plasmid

#227683

Purpose

To over express Phospho-dead Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567A

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1101

Plasmid

#235708

Purpose

Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
ATG14L (ATG14 Human)

Expression

Mammalian

Promoter

CAG and CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 V18D-OSF

Plasmid

#193042

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

V18D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 L61D-OSF

Plasmid

#193043

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

L61D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 F98D-OSF

Plasmid

#193044

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

F98D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CAPN7-V18D-mCherry

Plasmid

#193046

Purpose

Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Tight TRE

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CAPN7-C290S-mCherry

Plasmid

#193047

Purpose

Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Tight TRE

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 V18D,F98D (1-165)

Plasmid

#193034

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

V18D, F98D, Residues 1-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 316-366, C-Cys

Plasmid

#191234

Purpose

Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 316-366, contains nonnative C-terminus f…

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP4C 156-233

Plasmid

#108257

Purpose

Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP4C residues 156-233 (CHMP4C Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Contains nonnative Gly-Cys for fluorescent labeli…

Promoter

T7

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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