We narrowed to 4,418 results for: erf

1. pT2-hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #225999

   Purpose

   To establish stable dCas9-KRAB knock-in line

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   humanized dCas9-KRAB T2A Puro
   

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

   Plasmid

   #208046

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-dCas9-KRAB-PGK-HygR

   Plasmid

   #83890

   Purpose

   Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   humanized dead Cas9 KRAB
   aminoglycoside phosphotransferase from E. coli
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Human Ubiquitin C Promoter and mouse phosphoglyce…

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIZ-EGFP-MYO10-HMM

   Plasmid

   #87257

   Purpose

   Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   OpIE2

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOmniBac zz TEV YBBR TRF1

   Plasmid

   #185445

   Purpose

   Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Tags

   YBBR and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-dCas9-T2A-GFP

   Plasmid

   #193781

   Purpose

   Tricistronic expression of dCas9-EGFP-BSD

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   dead Cas9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with existing Cas9 promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB-CAGGS-dCas9

   Plasmid

   #110823

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-CohIII-CBM-HIS-ybbR

   Plasmid

   #60866

   Purpose

   Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   CohIII
   CBM
   

   Use

   Tags

   6xHIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Cysteine 63 to Serine in CBM module

   Promoter

   T7

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139094

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid

    #60865

    Purpose

    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    Xyl
    Xmod-DocIII
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Threonine 120 to Cysteine in Xylanase domain

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC8 human IFN-gamma

    Plasmid

    #17600

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    interferon-gamma cDNA (IFNG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2012

    Availability

    Academic Institutions and Nonprofits only

13. AAV-nEF-dCas9

    Plasmid

    #106430

    Purpose

    Expresses SpdCas9 from the nEF promoter in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    nEF-dCas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EF1a_TFAP2C_P2A_Hygro_Barcode

    Plasmid

    #120487

    Purpose

    Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX:PGRMC1

    Plasmid

    #136062

    Purpose

    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

    Depositor

    Francisco Domínguez Hernández

    Article

    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

    Insert

    Progesterone receptor membrane component 1 (PGRMC1 Human)
    

    Use

    Tags

    GST-PGRMC1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF1377

    Plasmid

    #141822

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF148 (ZNF148 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-MYO10-HMM

    Plasmid

    #87256

    Purpose

    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIBN-tagBFP-hTRF2

    Plasmid

    #103803

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-MYO10-HMM-Nanotrap

    Plasmid

    #87255

    Purpose

    Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    GFP-nanotrap

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

    Plasmid

    #163988

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M1457A

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge