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pCIS.MTRIA-NPFF

Plasmid

#184612

Purpose

For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPFF

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_zyxin

Plasmid

#83034

Purpose

Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_zyxin

Expression

Mammalian

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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GFPxm163

Plasmid

#193480

Purpose

Turn-off fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

Insert

GFPxm163

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA19k-RFP

Plasmid

#126105

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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Cox8x2-CeNL_pcDNA3

Plasmid

#89513

Purpose

Cyan color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-PGE

Plasmid

#184617

Purpose

For expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PGE

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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CeNL_pRSETb

Plasmid

#89510

Purpose

Cyan color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-YeNL_pcDNA3

Plasmid

#89519

Purpose

Yellow color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Article

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-OeNL_pcDNA3

Plasmid

#89523

Purpose

Orange color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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Cox8x2-OeNL_pcDNA3

Plasmid

#89526

Purpose

Orange color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD.Sigma32.I54N

Plasmid

#59982

Purpose

Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

Depositor

Jeffery Kelly

Article

Zhang et al ACS Chem Biol. 2014 Jul 22.

Insert

Sigma32 I54N

Expression

Bacterial

Mutation

I54N

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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LifeAct-mhYFP

Plasmid

#186524

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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LifeAct-mGarnet

Plasmid

#104307

Purpose

codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

LifeAct-mGarnet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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ReNL-H2B_pcDNA3

Plasmid

#89534

Purpose

Red color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTFr

Plasmid

#61192

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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mGarnet2-alpha-tubulin

Plasmid

#104318

Purpose

codes for a fusion of fluorescent protein mGarnet2 and alpha-tubulin, visualizes alpha-tubulin

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

mGarnet2-alpha-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LSSA12

Plasmid

#186545

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Expression

Mammalian

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTubuiln-Electra1

Plasmid

#184929

Purpose

Tubulin labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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