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pST_H2FLK5_CAEEL_199-435 (pBS0696)

Plasmid

#185215

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_199-435. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_199-435

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK5_CAEEL_542-800 (pBS0695)

Plasmid

#185214

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_542-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_542-800

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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KG#471

Plasmid

#110935

Purpose

Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1

Inserts

mig-13 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1884

Plasmid

#154332

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.15

Plasmid

#131373

Purpose

expression of MBP:PAA-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

paa-1 (paa-1 Nematode)

Expression

Bacterial

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJPL3[flp-6p::cmk-1::GFP]

Plasmid

#124613

Purpose

Used to confirm the expression of the trans-spliced GFP reporter in the expected ASE neurons

Depositor

Anne Brunet

Article

Lim et al J Neurosci. 2018 Jul 4;38(27):6114-6129.

Insert

[flp-6p::cmk-1::GFP]

Expression

Worm

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Tags

GFP(65C)

Expression

Worm

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEV9.03

Plasmid

#37495

Depositor

Geraldine Seydoux

Article

Voronina et al Development. 2010 May;137(9):1441-50. Ep

Insert

npp-16 (npp-16 Nematode)

Use

RNAi

Expression

Worm

Mutation

nts 162-673 of cds

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pGC244

Plasmid

#19672

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Available Since

Feb. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pGC75

Plasmid

#19640

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<1xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC79

Plasmid

#19655

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pBScMXT-Shaw3

Plasmid

#16113

Depositor

Larry Salkoff

Article

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Voltage-gated K+ channel (shw-3 Nematode)

Use

Xenopus oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pHW1221

Plasmid

#229873

Purpose

gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

QF-act-4 3' UTR::SEC

Expression

Worm

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHW622

Plasmid

#229868

Purpose

Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

reverse FRT3 (rFRT3)

Expression

Worm

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJH2106

Plasmid

#179250

Purpose

Pnmr-1::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2140

Plasmid

#179249

Purpose

Pacr-5::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-5

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH1897

Plasmid

#179247

Purpose

Pacr-2::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2165

Plasmid

#179222

Purpose

Punc-4::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7 (unc-7 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2164

Plasmid

#179223

Purpose

Pacr-5::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7 (unc-7 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-5

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJH1898

Plasmid

#179221

Purpose

Pacr-2::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7 (unc-7 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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