We narrowed to 32,140 results for: Eng

1. pVP082

   Plasmid

   #222034

   Purpose

   MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Greengate compatible cloning v…

   Tags

   Expression

   Mutation

   All unused Esp3i sites are domesticated

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pVP083

   Plasmid

   #222035

   Purpose

   MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Greengate compatible cloning v…

   Tags

   Expression

   Mutation

   All unused Esp3i sites are domesticated

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pRSET A-mEosEM-E

   Plasmid

   #226336

   Purpose

   Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

   Depositor

   Pingyong Xu

   Article

   Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

   Insert

   mEosEM-E
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-HaloKbp1a

   Plasmid

   #228793

   Purpose

   Chemigenetic indicator for potassium ion - high affinity

   Depositor

   Robert Campbell

   Article

   Cheng et al J Am Chem Soc. 2024 Nov 27. doi: 10.1021

   Insert

   HaloKbp1a
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBAD-HaloKbp1b

   Plasmid

   #228794

   Purpose

   Chemigenetic indicator for potassium ion - low affinity

   Depositor

   Robert Campbell

   Article

   Cheng et al J Am Chem Soc. 2024 Nov 27. doi: 10.1021

   Insert

   HaloKbp1b
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-HaloKbp1c

   Plasmid

   #228795

   Purpose

   Chemigenetic indicator for potassium ion - medium affinity

   Depositor

   Robert Campbell

   Article

   Cheng et al J Am Chem Soc. 2024 Nov 27. doi: 10.1021

   Insert

   HaloKbp1c
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_SV40-iBlue-pA

   Plasmid

   #200234

   Purpose

   Backbone for cloning iSBH-sgRNAs

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM3/cGata1

   Plasmid

   #83829

   Purpose

   Cloning of chicken GATA1

   Depositor

   Douglas Engel

   Article

   Yamamoto et al Genes Dev. 1990 Oct;4(10):1650-62.

   Insert

   Gata1 (GATA1 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFBOH-MHL_EML4:250-877

   Plasmid

   #210914

   Purpose

   Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   EML4:I250-A877 (EML4 Human)
   

   Use

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Insect

   Mutation

   K283E natural variant (VAR_031726), I382V Natural…

   Promoter

   Polyhedrin

   Available since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFBD-BirA_ATG16L1:303-607

    Plasmid

    #210942

    Purpose

    Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    ATG16L1:G303-Y607 (ATG16L1 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 4, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pFBD-BirA_LRRK2:2141-2527

    Plasmid

    #210901

    Purpose

    Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    LRRK2:T2141-E2527 (LRRK2 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    M2397T natural variant ( VAR_024965)

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pBacMam2-DiEx-LIC_LRRK2:2141-2527

    Plasmid

    #210900

    Purpose

    Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    LRRK2:T2141-E2527 (LRRK2 Human)
    

    Use

    Tags

    AENLYFQSHHHHHHHHHHDYKDDDDK and M

    Expression

    Insect

    Mutation

    M2397T natural variant ( VAR_024965)

    Promoter

    P10 and CMV

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFBD-BirA_CDC20:161-P477

    Plasmid

    #210888

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pFBD-BirA_CDC40:236-578

    Plasmid

    #210885

    Purpose

    Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC40:E236-W578 (CDC40 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMG-RIS

    Plasmid

    #225132

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEMG-GRIS

    Plasmid

    #225134

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pEMG-AIS

    Plasmid

    #225133

    Purpose

    For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET-pro-siRNA-V2

    Plasmid

    #111086

    Purpose

    For production of recombinant siRNAs (pro-siRNAs) in E. coli.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge