We narrowed to 4,700 results for: golden gate
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Plasmid#228597PurposeMammalian expression of mT-Sapphire, for MuSIC barcode constructionDepositorInsertmT-Sapphire
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-LSSmOrange
Plasmid#228600PurposeMammalian expression of LSSmOrange, for MuSIC barcode constructionDepositorInsertLSSmOrange
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTFP1
Plasmid#228602PurposeMammalian expression of mTFP1, for MuSIC barcode constructionDepositorInsertmTFP1
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mT-Sapphire
Plasmid#228616PurposeMammalian expression of mT-Sapphire and extra insert, for MuSIC barcode constructionDepositorInsertmT-Sapphire
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-miRFP670-TRF1
Plasmid#227378PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, WT (TERF1 Human)
UseLentiviralAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2368 MTK PB puro backbone
Plasmid#226673PurposeEmpty MTK PB cargo backbone marked with puromycin resistance.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-9YS-miRFP670-TRF1
Plasmid#227381PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-27YS-miRFP670-TRF1
Plasmid#227383PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
iLID-miRFP670-TRF1
Plasmid#227385PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric iLID (TERF1 Human)
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-15YS-miRFP670-TRF1
Plasmid#227382PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-5YS-miRFP670-TRF1
Plasmid#227380PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-3YS-miRFP670-TRF1
Plasmid#227379PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB50
Plasmid#226311PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-7
ExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)
Plasmid#221994PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
Tags10x His tag with E. coli maltose binding protein …ExpressionBacterialPromoterT7 promoter and T7 promoter-lacOAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5419_p3WJdB-glcO36
Plasmid#221193PurposeGlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminatorDepositorInsertT7 promoter-glcO36
UseSynthetic BiologyExpressionBacterialPromoterT7 promoter with downstream glcO36 operatorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd
Plasmid#216170PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd
Plasmid#216172PurposeExpress the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- Control- ZEB2.locus
UseLentiviralTagsEGFPAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2
Plasmid#216173PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
ExpressionYeastPromoterGAL7Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only