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We narrowed to 18,824 results for: nls

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Showing: 4101 - 4120 of 18824 results

  1. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

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  2. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139097-plasmid-map-sequence-id-273896

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  3. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  5. pCR1054

    Plasmid
    #71822
    Purpose
    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71822-plasmid-map-sequence-id-131019

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  6. pv6_BRD4(1_2)_BRD.1x

    Plasmid
    #260600
    Purpose
    Expresses the first and second bromodomains from BRD4 fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(1_2)_BRD.1x (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260600-plasmid-map-sequence-id-523813

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  7. pv6_BRD9_BRD.2x

    Plasmid
    #260604
    Purpose
    Expresses two copies of the bromodomain from BRD9 fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD9_BRD.2x (BRD9 Human)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260604-plasmid-map-sequence-id-523763

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  8. SGEP-sh-Hs-PHGDH-67

    Plasmid
    #188671
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188671-plasmid-map-sequence-id-375112

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  17. pSmart-EF1a-Nla-cas3

    Plasmid
    #178881
    Purpose
    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas3c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178881-plasmid-map-sequence-id-351754

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  18. pLenti EF1a mCherry-T2A-VP64-NtcA-2xNLS_SV40

    Plasmid
    #254310
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA chimera expression.
    Depositor
    Samuel McBrayer
    Publication
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254310-plasmid-map-sequence-id-508529

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  20. pv6_BRD4(2)_BRD.2x

    Plasmid
    #260597
    Purpose
    Expresses two copies of the second bromodomain from BAZ2a fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(2)_BRD.2x (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260597-plasmid-map-sequence-id-523811

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Showing: 4101 - 4120 of 18824 results