A transcriptional biosensor reveals mechanisms of alpha-ketoglutarate signaling to chromatin.

Sternisha AC, Li H, Gajendra K, Xiao Y, Zhao X, Traylor JI, Guo L, Jun JH, Fleishman M, Shipman T, Puliyappadamba VT, Kaphle P, Ouyang Q, Schmidt M, Shi DD, Savani MR, Tsai AC, Lee JH, Gordillo R, Garcia-Bermudez J, Kim YJ, Tso SC, Brautigam CA, Zacharias LG, Mathews TP, Xu L, Doench JG, Koduri V, Abdullah KG, Agathocleous M, Banaszynski LA, DeBerardinis RJ, Morrow EM, McBrayer SK
Science. 2026 Jul 16;393(6808):eadx8675. doi: 10.1126/science.adx8675. Epub 2026 Jul 16. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
254306	pLenti Neo glnA PCC 6803 5x sites native blastR-P2A-GFP	blastR-P2A-GFP reporter under regulation of CMV minimal promoter and glnA PCC 6803 tiled NtcA binding sites separated by native spacer elements. Requires NtcA chimera for reporter gene expression.
254307	pLenti Neo glnA PCC 6803 5x sites native GFP	GFP reporter under regulation of CMV minimal promoter and glnA PCC 6803 tiled NtcA binding sites separated by native spacer elements. Requires NtcA chimera for reporter gene expression.
254308	pLenti EF1a VP64-NtcA-2xNLS_SV40	Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid.
254309	pLenti EF1a VP64-NtcA_R90E-2xNLS_SV40	Constitutive expression of VP64- NtcA_R90E-2xNLS_SV40 for use in the inactive aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid.
254310	pLenti EF1a mCherry-T2A-VP64-NtcA-2xNLS_SV40	Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA chimera expression.
254311	pLenti EF1a mCherry-T2A-VP64-NtcA_R90E-2xNLS_SV40	Constitutive expression of VP64- NtcA_R90E-2xNLS_SV40 for use in the inactive aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA fusion expression.