Cas11 enables genome engineering in human cells with compact CRISPR-Cas3 systems.
Tan R, Krueger RK, Gramelspacher MJ, Zhou X, Xiao Y, Ke A, Hou Z, Zhang Y
Mol Cell. 2022 Feb 17;82(4):852-867.e5. doi: 10.1016/j.molcel.2021.12.032. Epub 2022 Jan 19. PubMed Article
Tan R, Krueger RK, Gramelspacher MJ, Zhou X, Xiao Y, Ke A, Hou Z, Zhang Y
Mol Cell. 2022 Feb 17;82(4):852-867.e5. doi: 10.1016/j.molcel.2021.12.032. Epub 2022 Jan 19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178878 | pSmart-EF1a-Nla-cas5 | Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag |
| 178879 | pSmart-EF1a-Nla-cas7 | Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag |
| 178880 | pSmart-EF1a-Nla-cas8 | Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag |
| 178881 | pSmart-EF1a-Nla-cas3 | Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag |
| 178882 | pSmart-EF1a-Nla-cas11 | Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag |
| 178883 | pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat | Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells. |
| 180213 | pET-NlaCascade-NLS-6xHis | Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli |
| 180214 | pET28-T7-NlaCas3-NLS-6xHis | Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli |
| 180215 | pACYC-T7-Nla-repeat-BbsI | For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli |
| 193752 | pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11 | human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH |