pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
(Plasmid
#178883)
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PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSmart HC Kan
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Backbone manufacturerLucigen
- Backbone size w/o insert (bp) 1812
- Total vector size (bp) 2166
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU6-Nla repeat-BbsI-Nla repeat
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gRNA/shRNA sequencen/a
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SpeciesSynthetic
- Promoter U6
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CAGTCCAGTTACGCTGGAGTC
- 3′ sequencing primer GGTCAGGTATGATTTAAATGGTCAGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat was a gift from Yan Zhang (Addgene plasmid # 178883 ; http://n2t.net/addgene:178883 ; RRID:Addgene_178883) -
For your References section:
Cas11 enables genome engineering in human cells with compact CRISPR-Cas3 systems. Tan R, Krueger RK, Gramelspacher MJ, Zhou X, Xiao Y, Ke A, Hou Z, Zhang Y. Mol Cell. 2022 Feb 17;82(4):852-867.e5. doi: 10.1016/j.molcel.2021.12.032. Epub 2022 Jan 19. 10.1016/j.molcel.2021.12.032 PubMed 35051351
Map uploaded by the depositor.
