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pHU6_gRNA_Ch10-for1

Plasmid

#81210

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev2

Plasmid

#81207

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev1

Plasmid

#81208

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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shCDK6(2)

Plasmid

#73553

Purpose

shRNA(2) against CDK6

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23107

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #3-RFP

Plasmid

#81077

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23101

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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shCDK6(1)

Plasmid

#73552

Purpose

shRNA(1) against CDK6

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

Plasmid

#65662

Purpose

FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-NtermN2

Plasmid

#51446

Purpose

Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K150Q and K153Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-NtermN2

Plasmid

#51447

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N2 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q and K153Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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MR015-PKP2 Left TALEN_NN

Plasmid

#49451

Purpose

Left TALEN for PKP2 with NN

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Expression

Mammalian

Available Since

Jan. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pPROBE'-gfp[ASV]

Plasmid

#40167

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pPROBE'-gfp[tagless]

Plasmid

#40165

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pPROBE-KT'

Plasmid

#37817

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
hU6-pegRNA-EF-1α-Cre Template

Plasmid

#196036

Purpose

The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Promoter

hU6 and EF-1-alpha

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR 223 VPS4B

Plasmid

#169320

Purpose

Gateway entry vector for human VPS4B ORF

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

VPS4B (VPS4B Human)

Use

Gateway Cloning

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV77

Plasmid

#244906

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV77

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG--uMASS_LF

Plasmid

#209763

Purpose

Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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