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Addgene

We narrowed to 16,609 results for: IGH@

Showing: 10551 - 10600 of 16609 results
  1. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pBT3019

    Plasmid
    #122541
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2
    Depositor
    Insert
    Pnhx-2_CemOrange2::SKL
    Use
    Tags
    SKL
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pYPQ269C2

    Plasmid
    #164716
    Purpose
    Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing
    Depositor
    Insert
    hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible haid-cbe_v04 entry clo…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. p1.2-Zeo-eGFP

    Plasmid
    #162743
    Purpose
    Fluorescent reporter for CHO cells expression studies
    Depositor
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

    Plasmid
    #207613
    Purpose
    vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells
    Depositor
    Insert
    mGreenLantern
    Use
    AAV
    Tags
    KASH
    Expression
    Mutation
    Promoter
    mouse Pcp2 promoter (L7-6)
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pLV-REPORT(EF1a)-TTN

    Plasmid
    #172327
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pYPQ269A2

    Plasmid
    #164714
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing
    Depositor
    Insert
    rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible rapobec1-cbe_v04 entry…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. GFP-BICD2N

    Plasmid
    #64070
    Purpose
    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)
    Depositor
    Insert
    BICD2N (1-400) (Bicd2 Mouse)
    Use
    Tags
    GFP and His-ZZ-TCS
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Promoter
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pMDC123SB-OsMIR390-B/c

    Plasmid
    #61466
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    Insert
    OsMIR390-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    2x35S
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. pYPQ265

    Plasmid
    #164712
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing
    Depositor
    Insert
    rAPOBEC1-zCas9(D10A)-UGI
    Use
    CRISPR; Gateway compatible rapobec1-cbe_v01 entry…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 10551 - 10600 of 16609 results