We narrowed to 6,361 results for: nls
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Plasmid#107136PurposecGAL-C driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
ExpressionWormPromoterC. elegans eft-3 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL2669 (pTnsABf_Vch)
Plasmid#200897PurposeExpresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.DepositorInsertVchTnsA-TnsB
TagsInternal NLS between TnsA-TnsB fusionExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)Mutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
TagsNLS (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate-VP64
Plasmid#47389PurposeExpression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFLAG, HA, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-max
Plasmid#198888PurposeExpress A3Bctd-max BE4max-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
Tags6x His and NLSExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-CP1028
Plasmid#138160PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
EE-BE4max
Plasmid#138158PurposeC-to-T base editorDepositorInsertrAPOBEC1(EE)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R126E + R132E; within Cas9 D10APromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1209
Plasmid#44491DepositorInsertMinimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]
UseGateway expression cloneAvailable SinceApril 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantPromoter35SPPDK promoterAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GWF297
Plasmid#133612PurposeTET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFPDepositorInsertTET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
ExpressionMammalianAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-SsAPOBEC3B R54Q
Plasmid#138344PurposeMammalian expression plasmid for BE4-SsAPOBEC3B R54QDepositorInsertBE4-SsAPOBEC3B R54Q
UseSynthetic BiologyTagsBP-NLSPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM124: pAAV-EFS-CasRx-VEGFA presgRNA
Plasmid#166872PurposeAAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editingDepositorInsertsU6-VEGFA presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianPromoterEFS and U6Available SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-RrA3F F130L
Plasmid#138341PurposeMammalian expression plasmid for BE4-RrA3F F130LDepositorInsertBE4-RrA3F F130L
UseSynthetic BiologyTagsBP-NLSPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)
Plasmid#107135PurposecGAL-C driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
ExpressionWormPromoterC. elegans myo-2 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTX209
Plasmid#89269PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02DepositorInsertOsPDS-gRNA01 and OsPDS-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV MN (eGFP)
Plasmid#89932PurposeExpresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertMN
TagsHAExpressionMammalianMutationM.SssI residues 2-272 fragmentPromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only